Intergenic transcription by RNA polymerase II coordinates Pol IV and Pol V in siRNA-directed transcriptional gene silencing in Arabidopsis.

Genes & Development
Binglian ZhengXuemei Chen

Abstract

Intergenic transcription by RNA Polymerase II (Pol II) is widespread in plant and animal genomes, but the functions of intergenic transcription or the resulting noncoding transcripts are poorly understood. Here, we show that Arabidopsis Pol II is indispensable for endogenous siRNA-mediated transcriptional gene silencing (TGS) at intergenic low-copy-number loci, despite the presence of two other polymerases-Pol IV and Pol V-that specialize in TGS through siRNAs. We show that Pol II produces noncoding scaffold transcripts that originate outside of heterochromatic, siRNA-generating loci. Through these transcripts and physical interactions with the siRNA effector protein ARGONAUTE4 (AGO4), Pol II recruits AGO4/siRNAs to homologous loci to result in TGS. Meanwhile, Pol II transcription also recruits Pol IV and Pol V to different locations at heterochromatic loci to promote siRNA biogenesis and siRNA-mediated TGS, respectively. This study establishes that intergenic transcription by Pol II is required for siRNA-mediated TGS, and reveals an intricate collaboration and division of labor among the three polymerases in gene silencing.

References

May 4, 2002·Science·Philipp KapranovThomas R Gingeras
Aug 24, 2002·Science·Thomas A VolpeRobert A Martienssen
Aug 29, 2002·The EMBO Journal·Andrew HamiltonDavid Baulcombe
Dec 19, 2003·Current Biology : CB·Xiaofeng CaoSteven E Jacobsen
Mar 17, 2004·PLoS Biology·Zhixin XieJames C Carrington
May 4, 2004·Current Biology : CB·Tatsuo KannoAntonius J M Matzke
Feb 5, 2005·Science·A J HerrD C Baulcombe
Jun 1, 2005·Nature Genetics·Tatsuo KannoAntonius J M Matzke
Feb 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Carrie Anne Davis, Manuel Ares
May 26, 2006·The EMBO Journal·Bruno HuettelMarjori Matzke
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xiaoyu ZhangSteven E Jacobsen
May 19, 2007·Science·Philipp KapranovThomas R Gingeras
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rebecca A MosherDavid C Baulcombe
Dec 17, 2008·Nature Structural & Molecular Biology·Linfeng HuangDavid C Baulcombe
Jan 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sylvie LahmyThierry Lagrange
Jan 27, 2009·Nature·Zhenyu XuLars M Steinmetz
Apr 21, 2009·Nature Genetics·Andrzej T WierzbickiCraig S Pikaard
Jul 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ahmad M KhalilJohn L Rinn

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Citations

Oct 19, 2010·Plant Cell Reports·Youli YaoIgor Kovalchuk
Jul 5, 2012·Planta·Cecilia Contreras-CubasAlejandra A Covarrubias
Feb 16, 2011·Cell Research·Xin-Jian HeJian-Kang Zhu
May 13, 2010·Heredity·F K Teixeira, V Colot
Feb 10, 2010·Nature Reviews. Genetics·Julie A Law, Steven E Jacobsen
Jul 23, 2011·Nature Reviews. Molecular Cell Biology·Jeremy R Haag, Craig S Pikaard
Aug 7, 2012·Nature Structural & Molecular Biology·Xuehua ZhongSteven E Jacobsen
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xiaojie LiZhizhong Gong
Jul 7, 2011·The Journal of Biological Chemistry·Xizhe Zhang, John J Rossi
Jul 14, 2010·Nucleic Acids Research·Padmanabhan ChellappanHailing Jin
May 5, 2010·Genes & Development·Hailing Jin, Jian-Kang Zhu
Aug 17, 2012·Genes & Development·Huiming Zhang, Jian-Kang Zhu
Dec 28, 2012·Genome Research·Jonathan I GentR Kelly Dawe
Mar 5, 2013·BMC Genomics·Anupma SharmaGernot G Presting
Jul 2, 2010·Silence·Rebecca A MosherPadubidri V Shivaprasad
May 10, 2012·PLoS Genetics·Cui-Jun ZhangXin-Jian He
Jun 19, 2013·PloS One·Olga PontesPedro Costa-Nunes
Jul 28, 2013·International Journal of Molecular Sciences·Mikhail M Pooggin
Sep 10, 2013·Trends in Cell Biology·Donna M Bond, David C Baulcombe
May 17, 2014·Biology·Chao-Feng Huang, Jian-Kang Zhu
May 23, 2014·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Pedro Costa-NunesOlga Pontes
May 9, 2014·Integrative and Comparative Biology·Ashley L Jones, Sibum Sung
May 9, 2014·Nature Reviews. Genetics·Marjori A Matzke, Rebecca A Mosher
Nov 5, 2013·Biochimica Et Biophysica Acta·Angel Emilio Martínez de AlbaHervé Vaucheret
Jan 31, 2015·National Science Review·Yuanyuan Zhao, Xuemei Chen

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