Interindividual Differences in Human Intestinal Microbial Conversion of (-)-Epicatechin to Bioactive Phenolic Compounds

Journal of Agricultural and Food Chemistry
Chen LiuIvonne M C M Rietjens

Abstract

To quantify interindividual differences in the human intestinal microbial metabolism of (-)-epicatechin (EC), in vitro anaerobic incubations with fecal inocula from 24 healthy donors were conducted. EC-derived colonic microbial metabolites were qualitatively and quantitively analyzed by liquid chromatography triple quadrupole mass spectrometry (LC-TQ-MS) and liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS). Quantitative microbiota characterization was achieved by 16S rRNA analysis. The results obtained show 1-(3',4'-dihydroxyphenyl)-3-(2″,4″,6″-dihydroxyphenyl)-2-propanol (3,4-diHPP-2-ol) and 5-(3',4'-dihydroxyphenyl)-γ-valerolactone (3,4-diHPV) to be key intermediate microbial metabolites of EC and also revealed the substantial interindividual differences in both the rate of EC conversion and the time-dependent EC metabolite pattern. Furthermore, substantial differences in microbiota composition among different individuals were detected. Correlations between specific microbial phylotypes and formation of certain metabolites were established. It is concluded that interindividual differences in the intestinal microbial metabolism of EC may contribute to interindividual differences in potential health effects of...Continue Reading

References

Sep 1, 1984·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·G Groenewoud, H K Hundt
May 1, 1997·Chemical & Pharmaceutical Bulletin·M R MeselhyM Hattori
Apr 17, 2001·Chemical Research in Toxicology·H M AwadI M Rietjens
Apr 20, 2001·Journal of Applied Microbiology·R Puupponen-PimiäK M Oksman-Caldentey
Feb 14, 2003·Lancet·Francisco Guarner, Juan-R Malagelada
May 13, 2003·Clinical Pharmacokinetics·Edzard SchwedhelmRainer H Böger
Jan 11, 2005·The American Journal of Clinical Nutrition·Claudine ManachChristian Rémésy
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Nov 23, 2006·Chemical Research in Toxicology·Yee Y Lee-HilzIvonne M C M Rietjens
Nov 4, 2008·Molecular Nutrition & Food Research·Angélique StalmachAlan Crozier
Jun 24, 2009·Chemical Research in Toxicology·Małgorzata Muzolf-PanekIvonne M C M Rietjens
Nov 26, 2009·Molecular Nutrition & Food Research·Angélique StalmachAlan Crozier
Nov 28, 2009·Molecular Nutrition & Food Research·Stavroula StoupiMichael N Clifford
Jan 1, 2010·Journal of Agricultural and Food Chemistry·Suri RoowiAlan Crozier
Jan 19, 2010·Nutrition·Daniele Del RioFurio Brighenti
Oct 12, 2010·Biotechnology Journal·Markus SchantzElke Richling
Apr 5, 2011·Journal of Applied Microbiology·M KutscheraA Braune
Jun 1, 2012·Journal of Agricultural and Food Chemistry·Fernando Sánchez-PatánBegoña Bartolomé
Apr 30, 2013·Journal of Agricultural and Food Chemistry·Akiko Takagaki, Fumio Nanjo
Nov 1, 2013·The American Journal of Clinical Nutrition·Michael N CliffordAlan Crozier
May 20, 2015·Molecular Nutrition & Food Research·Ana Rodriguez-MateosJeremy P E Spencer
May 27, 2015·Diagnostic Microbiology and Infectious Disease·Ivan BruknerAndre Dascal
Jun 9, 2016·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Jan F Stevens, Claudia S Maier
Mar 23, 2017·Biochemical Pharmacology·Gary Williamson, Michael N Clifford
Apr 19, 2017·Gut Microbes·Ilias LagkouvardosThomas Clavel
May 10, 2017·Biochemical Pharmacology·Juan Carlos EspínFrancisco A Tomás-Barberán
Jun 24, 2017·Science·Nitzan KoppelEmily P Balskus
Nov 12, 2017·Molecular Aspects of Medicine·Gina BorgesAlan Crozier
Feb 9, 2018·Molecular Aspects of Medicine·Cesar G FragaMonica Galleano
Jul 17, 2018·Toxicology and Applied Pharmacology·C BehrB van Ravenzwaay
Aug 1, 2018·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Judy L BoltonBirgit M Dietz

❮ Previous
Next ❯

Citations

Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·P Matthew Joyner
Sep 14, 2021·Critical Reviews in Food Science and Nutrition·Qiqiong Li, Tom Van de Wiele
Sep 14, 2021·The British Journal of Nutrition·Mostafa Hassan BakyMohamed A Farag
Nov 24, 2021·Pflügers Archiv : European journal of physiology·Ezequiel J HidMonica Galleano

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
PCA

Software Mentioned

Graphpad Prism
XLSTAT
Labsolutions
HyStar
ChemDraw

Related Concepts

Related Feeds

Bacteriotherapy (ASM)

Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.

Bacteriotherapy

Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.