Interkingdom signaling: integration, conformation, and orientation of N-acyl-L-homoserine lactones in supported lipid bilayers.

Langmuir : the ACS Journal of Surfaces and Colloids
Christoph BarthPatrick Koelsch

Abstract

N-Acyl-L-homoserine lactones (AHLs) are small cell-to-cell signaling molecules involved in the regulation of population density and local gene expression in microbial communities. Recent evidence shows that contact of this signaling system, usually referred to as quorum sensing, to living eukaryotes results in interactions of AHL with host cells in a process termed "interkingdom signaling". So far details of this process and the binding site of the AHLs remain unknown; both an intracellular and a membrane-bound receptor seem possible, the first of which requires passage through the cell membrane. Here, we used sum-frequency-generation (SFG) spectroscopy to investigate the integration, conformation, orientation, and translocation of deuterated N-acyl-L-homoserine lactones (AHL-d(n)) with varying chain length (8, 12, and 14 C atoms) in lipid bilayers consisting of a 1:1 mixture of POPC:POPG supported on SiO(2) substrates (prepared by vesicle fusion). We found that all AHL-d(n) derivatives are well-ordered within the supported lipid bilayer (SLB) in a preferentially all-trans conformation of the deuterated alkyl chain and integrated into the upper leaflet of the SLB with the methyl terminal groups pointing downward. For the bilaye...Continue Reading

References

Jan 1, 1987·Annual Review of Microbiology·J W CostertonT J Marrie
Jun 1, 1984·Journal of Clinical Microbiology·T J Marrie, J W Costerton
May 21, 1999·Science·J W CostertonE P Greenberg
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Jan 4, 2001·Microbes and Infection·K P RumbaughA N Hamood
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·Clay Fuqua, E Peter Greenberg
Feb 26, 2003·Journal of Biomedical Materials Research. Part a·Ann-Sofie AnderssonBengt Kasemo
Jul 9, 2004·Journal of the American Chemical Society·Jin Liu, John C Conboy
Jul 22, 2004·Journal of the American Chemical Society·Jin Liu, John C Conboy
Apr 9, 2005·Environmental Microbiology·Mario JuhasBurkhard Tümmler
Feb 16, 2006·International Journal of Medical Microbiology : IJMM·Martin Schuster, E Peter Greenberg
Feb 24, 2006·Journal of the American Chemical Society·Xiaoyun ChenZhan Chen
Mar 10, 2006·Biochimica Et Biophysica Acta·Xiaoyun Chen, Zhan Chen
Apr 6, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Ralf P RichterAlain R Brisson
Sep 22, 2006·Infection and Immunity·Sabine ZimmermannGertrud Maria Hänsch
Oct 2, 2007·Journal of the American Chemical Society·Xiaoyun ChenZhan Chen
Jan 17, 2008·Nature Reviews. Microbiology·David T Hughes, Vanessa Sperandio
Dec 5, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Caitlin HowellPatrick Koelsch
Jan 15, 2009·Journal of the American Chemical Society·Trang T NguyenJohn C Conboy
Sep 5, 2009·The Journal of Physical Chemistry. B·Khoi Tan NguyenZhan Chen
Mar 11, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Volker KurzPatrick Koelsch
Apr 17, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Peter J N KettPaul B Davies
Sep 1, 2008·Biointerphases·Caitlin HowellPatrick Koelsch
May 28, 2010·The Journal of Physical Chemistry. B·Khoi Tan NguyenZhan Chen
Jul 2, 2010·The Review of Scientific Instruments·Dominique VerreaultPatrick Koelsch
Oct 12, 2010·Journal of the American Chemical Society·Khoi Tan NguyenZhan Chen
May 21, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Pei YangZhan Chen
Jun 30, 2011·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Ting WangZhan Chen

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Citations

Jan 20, 2016·Pathogens and Disease·Matthias Martin GaidaGertrud Maria Hänsch
Aug 8, 2015·ACS Nano·Merve DoğangünFranz M Geiger
Jun 22, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Dan Lis, Francesca Cecchet
Mar 11, 2017·The ISME Journal·Masanori ToyofukuNobuhiko Nomura
Mar 14, 2017·Clinical Biochemistry·Jason Y Park, Larry J Kricka
Apr 26, 2019·Bioscience, Biotechnology, and Biochemistry·Masanori Toyofuku
Jul 30, 2017·Science China. Life Sciences·Jinhong KanYantao Jia
Nov 7, 2020·FEMS Microbiology Ecology·Abhishek Shrestha, Adam Schikora
Jul 21, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Curran G GahanDavid M Lynn
Oct 15, 2013·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Pei YangZhan Chen
Jun 23, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Tamás KeszthelyiLászló Héja
Apr 9, 2013·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Pei YangZhan Chen
Oct 5, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Curran G GahanDavid M Lynn

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