PMID: 8950567Oct 1, 1996Paper

Interlaboratory comparison of polymerase chain reaction for the detection of Toxoplasma gondii DNA added to samples of amniotic fluid

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
E C GuyP Ambroise-Thomas

Abstract

To investigate the accuracy of the polymerase chain reaction (PCR) method for the detection of Toxoplasma gondii in clinical specimens, aliquots of amniotic fluid to which known amounts of Toxoplasma gondii DNA had been added were tested by five European Centres. Four laboratories were able to detect DNA at levels equivalent to ten tachyzoites or less, including two that detected DNA equivalent to a single parasite. Two laboratories erroneously found one of eight negative control samples to be positive. These findings confirm that the high level of sensitivity associated with the PCR method can be readily achieved under routine laboratory conditions, but they also underscore the potential for both false-positive and false-negative findings to occur. Furthermore, the results confirm the urgent need for an external quality assurance scheme to support laboratories employing PCR in a clinical context for the detection of Toxoplasma gondii.

References

Dec 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A RothK Janitschke
Nov 1, 1992·Journal of Clinical Microbiology·S F ParmleyJ S Remington
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Jul 1, 1993·Journal of Clinical Microbiology·J Dupouy-CametF Derouin

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Citations

Jan 18, 2006·Folia Microbiologica·Z CermákováL Plísková
Aug 3, 2010·Transactions of the Royal Society of Tropical Medicine and Hygiene·Carolina C CorreiaVláudia M A Costa
Mar 10, 2001·International Journal for Parasitology·E PetersenI Reiter-Owona
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Patrick Bastien
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·S BretagneC Cordonnier
Sep 18, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·P A JenumB Stray-Pedersen
Apr 9, 2004·Journal of Clinical Microbiology·Elisabeth ChabbertPatrick Bastien
Nov 13, 2008·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·F A Habib
Dec 9, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Alison E HillmanR C Andrew Thompson
Jan 18, 2006·Veterinary Parasitology·Franz J Conraths, Gereon Schares
May 3, 2001·Obstetrical & Gynecological Survey·J L JonesR Gibbs
Mar 4, 2000·Journal of Clinical Microbiology·J G GarwegM Boehnke
Sep 18, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S JalalUNKNOWN ESCMID Study Group on Toxoplasmosis

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