Interlaboratory reproducibility of Etest amphotericin B and caspofungin yeast susceptibility testing and comparison with the CLSI method.

Journal of Clinical Microbiology
S RanqueS Bertout

Abstract

This study aimed to assess the interlaboratory reproducibility at four university hospital laboratories in the southeast region of France of the Etest technique for the determination of caspofungin (CAS) and amphotericin B (AMB) MICs and to compare it to the CLSI broth microdilution reference method. Consecutive clinical yeast isolates (n = 198) were included in the study. AMB and CAS MICs were read at 24 and 48 h. Interlaboratory reproducibility was estimated by using (i) an intraclass correlation coefficient (ICC), (ii) essential agreement (EA), and (iii) categorical agreement (CA). For Etest interlaboratory reproducibility for CAS, ICCs were 0.80 (95% confidence interval [CI], 0.76 to 0.84) and 0.81 (95% CI, 0.77 to 0.85) at 24 and 48 h, respectively. For AMB, the ICCs were 0.51 (95% CI, 0.43 to 0.58) and 0.69 (95% CI, 0.63 to 0.74) at 24 and 48 h, respectively. At 48 h, the between-center EAs ranged from 94.4 to 99.0% for both antifungals. For the comparison of the CLSI method and the Etest, the between-technique ICCs were 0.69 (95% CI, 0.63 to 0.74) and 0.62 (95% CI, 0.55 to 0.68) for CAS and AMB, respectively. The EAs ranged from 76.5 to 98.5% for CAS and from 90.3 to 97.4% for AMB according to the centers. CAs ranged fro...Continue Reading

References

Dec 15, 1994·Statistics in Medicine·R Müller, P Büttner
Jan 13, 1999·Diagnostic Microbiology and Infectious Disease·M A PfallerA Bolmström
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Luis Ostrosky-ZeichnerJeannette Lee
Mar 3, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mikkel Krogh-MadsenJenny Dahl Knudsen
Jan 25, 2007·Journal of Medical Microbiology·Eiman M MokaddasZia U Khan
Sep 22, 2007·Medical Mycology·Sevtap Arikan
Aug 14, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·W W Hope, G L Drusano
Sep 11, 2009·Current Opinion in Infectious Diseases·David S Perlin
Sep 26, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E DannaouiS Bretagne
Oct 7, 2009·Expert Review of Anti-infective Therapy·Maria Teresa FeraAngelina De Sarro

❮ Previous
Next ❯

Citations

Jun 10, 2015·Antioxidants & Redox Signaling·Michael BlatzerDoris Wilflingseder
Jun 24, 2015·Canadian Journal of Microbiology·Débora de Souza Collares Maia Castelo-BrancoTereza de Jesus Pinheiro Gomes Bandeira
Sep 24, 2013·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Paula Volpato SanitáCarlos Eduardo Vergani
Aug 28, 2015·Revista Da Sociedade Brasileira De Medicina Tropical·Fábio Silvestre AtaidesMaria do Rosário Rodrigues Silva
Apr 24, 2020·Expert Review of Anti-infective Therapy·Ludwig Knabl, Cornelia Lass-Flörl
Jan 11, 2019·PloS One·Andrew A Heller, Dana M Spence
Dec 29, 2020·Journal of Veterinary Research·Ireneusz SokółAndrzej Gaweł
Oct 17, 2015·Indian Journal of Medical Microbiology·S R MotukupallyS Sharma

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

© 2021 Meta ULC. All rights reserved