Interlaboratory Validation of the in vitro Eye Irritation Tests for Cosmetic Ingredients. (4) Haemoglobin Denaturation Test

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M HataoY Ohno

Abstract

Interlaboratory validation of the haemoglobin denaturation (HD) test on 38 cosmetic ingredients was conducted by five to eight participating laboratories. The HD test was evaluated as an alternative method to the Draize eye irritation test (Draize test) based on three indices of protein denaturation: the test substance concentration that induces 50% HD of the positive control (RDC(50)), a relative HD rate at 1% of the test substance (1%RDR) and a relative change in maximum absorption wavelength (1% lambdamax). The coefficients of variation associated with a positive HD test among the participating laboratories were within an acceptable range for practical application. The in vitro test results were in relatively good agreement with the Draize test. The correlation coefficient (r) between the in vivo maximal average Draize total score (MAS) and log (RDC(50)), 1%RDR and 1% lambdamax were -0.91, 0.67 and 0.79, respectively. The results revealed several limitations associated with the HD test: (1) the HD test cannot be applied to coloured test substances with a strong absorption, around 418nm; (2) water-insoluble test substances cannot be evaluated by RDC(50) or 1%RDR; (3) the HD test cannot be applied to strong acids that exceed t...Continue Reading

References

Feb 1, 1984·International Journal of Cosmetic Science·K MiyazawaT Mitsui
Apr 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T HayashiS Kato
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·V C GordonH C Bergman
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P CatrouxM Cottin

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Citations

Oct 22, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M G SoniG A Burdock
Aug 19, 2007·Journal of Pharmaceutical Sciences·M P Vinardell, M Mitjans
Jul 8, 2008·Regulatory Toxicology and Pharmacology : RTP·Montserrat MitjansM Pilar Vinardell
Mar 19, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Chantra EskesNathan Wilt
Apr 12, 2006·Oncogene·S S ShethA J Lusis
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Chantra EskesValérie Zuang
Dec 15, 2004·Alternatives to Laboratory Animals : ATLA·Ludwig A Hothorn

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