Interlaboratory Validation of the In Vitro Eye Irritation Tests for Cosmetic Ingredients. (6) Evaluation of MATREX((TM))

Toxicology in Vitro : an International Journal Published in Association with BIBRA
J OhuchiY Ohno

Abstract

MATREX(TM) is a test system for evaluating eye irritation potential, using the living dermal model (LDM). The LDM consists of normal human dermal fibroblasts in a contracted collagen lattice, which eventually forms a three-dimensional structure. This system has several advantages. It can be applied to insoluble substances and does not require sterile conditions for operation. In the present study, MATREX was introduced as an alternative to the Draize eye irritation test (Draize test) for cosmetics ingredients. MATREX was evaluated through a three-phase series interlaboratory validation as part of a joint project of the National Institute of Health Sciences (NIHS) and Japan Cosmetic Industry Association (JCIA). Toxicity for LDM was mainly evaluated by cytotoxicity, the indicator was EC(50) (concentration that inhibits the viability of the cell to 50% of control) value. Additionally, MATREX score indicating the grade of cytotoxicity was also introduced in the third phase of the validation study. Both test procedures were controlled under the same standard operating procedure (SOP), at all the participating laboratories. A total of 39 test substances both water-soluble and -insoluble were examined. LDM was applicable to almost all...Continue Reading

References

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Citations

Sep 2, 2009·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Sanae MatsudaMasahiro Iwaki
Jun 6, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sanae MatsudaMasahiro Iwaki
Jul 31, 2016·BMC Ophthalmology·Nicholas CookIan A Cree
Sep 15, 2006·Neuroscience Research·Glaucie Jussilane AlvesJoão Palermo-Neto
Aug 30, 2007·The Journal of Pharmacy and Pharmacology·Terry B ErnestJames L Ford
Dec 15, 2016·Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research·Priyanka P MakhijaVaibhav Awinashe
Dec 15, 2004·Alternatives to Laboratory Animals : ATLA·Ludwig A Hothorn
Jan 22, 2009·Alternatives to Laboratory Animals : ATLA·Shigenobu HaginoHiroshi Itagaki
May 29, 2010·Alternatives to Laboratory Animals : ATLA·Shigenobu HaginoHiroshi Itagaki

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