Interlaboratory validation of the in vitro eye irritation tests for cosmetic ingredients. (8) Evaluation of cytotoxicity tests on SIRC cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
N TaniY Ohno

Abstract

Two common assays, the neutral red uptake assay (SIRC-NRU) and the crystal violet staining assay (SIRC-CVS), were evaluated as alternatives to the Draize eye irritation test (Draize test).The cytotoxicity of thirty-eight cosmetic ingredients as well as a physiological saline solution was determined on SIRC cells at five to seven laboratories. SIRC-NRU and SIRC-CVS were performed according to the common standard operating procedure (SOP). The 50% effective concentration (EC(50)) was determined for each ingredient. The EC(50) of SIRC-CVS was similar to that of SIRC-NRU, showing a strong correlation (r=0.995). The coefficient of variation (CV) of EC(50) which represents the interlaboratory reproducibility of SIRC-NRU was 32.1%, whereas that of SIRC-CVS was 32.8%. The logarithmically transformed EC(50) values showed a strong correlation with the maximal average Draize total score (MAS) (SIRC-NRU: r=-0.816 (n=30), SIRC-CVS: r=-0.805 (n=29)). Both methods could be applied to water-insoluble substances and dyes. However, strong acids, alkanolamines and alcohols had a tendency to deviate from the linear regression lines which were obtained from the in vivo and in vitro data for both methods in the present study. These results suggest t...Continue Reading

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Citations

Oct 22, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M G SoniG A Burdock
Sep 2, 2009·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Sanae MatsudaMasahiro Iwaki
Jun 7, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Toshiaki TakezawaPi-Chao Wang
Jun 6, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sanae MatsudaMasahiro Iwaki
Feb 25, 2010·Cutaneous and Ocular Toxicology·Yutaka TakahashiNaohiro Nishiyama
Feb 6, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yutaka TakahashiNaohiro Nishiyama
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Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Monica Helena Monteiro do NascimentoDaniele Ribeiro de Araujo

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