Interlaboratory validation of the in vitro eye irritation tests for cosmetic ingredients (9). Evaluation of cytotoxicity test on HeLa cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
K ChibaY Ohno

Abstract

The cytotoxicity test using MTT on HeLa cells (HeLa-MTT) was evaluated as an alternative method to the Draize eye irritation test (Draize test) by six to eight laboratories. The 50% inhibition concentration (EC(50)) for MTT reduction was calculated. A total of thirty-nine test chemicals were examined. The average interlaboratory coefficient of variation (CV) of the present assay was 25%. Comparison of the in vitro test results with those of the Draize test revealed a correlation coefficient of -0.799 between the log(EC(50)) value and the maximum average total score (MAS) for a 10% solution or suspension. The following characteristics of HeLa-MTT have become apparent through this validation: (1) HeLa-MTT could be applied to all substances including water-insoluble substances, esters, a colour additive, and a substance which is known to directly reduce MTT; (2) there is good interlaboratory reproducibility and strong correlation between HeLa-MTT and Draize MAS; (3) results for strong acids, alkanolamines and alcohols (lower mono-ol) clearly deviate from other samples with respect to the correlation between HeLa-MTT and Draize MAS. These results suggest that HeLa-MTT may be useful for predicting the Draize MAS if definite criteria...Continue Reading

References

Feb 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H North-RootJ E Heinze
Aug 1, 1985·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J Selling, B Ekwall
Oct 1, 1982·International Journal of Cosmetic Science·M C Scaife
Apr 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L VianJ P Cano
Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E BorenfreundN Martin-Alguacil
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Citations

Sep 2, 2009·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Sanae MatsudaMasahiro Iwaki
Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·Doris RengstlWerner Kunz
Sep 30, 2014·Drug Development and Industrial Pharmacy·Francisca RodriguesMaria Beatriz P P Oliveira
Feb 10, 2009·Colloids and Surfaces. B, Biointerfaces·N VlachyW Kunz
Mar 7, 2007·Dental Materials Journal·Satoshi JinnoToshihide Noguchi
Dec 15, 2004·Alternatives to Laboratory Animals : ATLA·Ludwig A Hothorn
Jan 22, 2009·Alternatives to Laboratory Animals : ATLA·Shigenobu HaginoHiroshi Itagaki
Jun 29, 2018·Chemico-biological Interactions·Artur Christian Garcia da SilvaMarize Campos Valadares

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