Interleukin-1 alpha, interleukin 6 and tumor necrosis factor alpha increase the synthesis and expression of the functional alternative and terminal complement pathways by human umbilical vein endothelial cells in vitro

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
V BergeK E Berge

Abstract

The proinflammatory cytokines interleukin 1 alpha (IL-1 alpha), tumor necrosis factor alpha (TNF alpha) and interleukin 6 (IL-6) modulate the synthesis of complement factors B and C3 by endothelial cells (EC), and are considered to play an important role in the development of sepsis. By using agarose beads activating the alternative pathway of complement, we wanted to study the net effect of these cytokines on EC synthesis of the alternative and terminal pathways, measured by binding of anti-C3c and anti-TCC (terminal complement complex) antibodies to beads kept with the EC. Addition of IL-1 alpha and TNF alpha at concentrations of 50 and 100 U/ml resulted in a significant increase in binding of these antibodies to co-incubated beads, most pronounced for anti-C3c. IL-6 from 50-200 U/ml resulted in a stronger (two to fourfold) binding for both antibodies compared to experiments with IL-1 alpha and TNF. However, increased concentrations of IL-1 alpha (200 U/ml) and IL-6 (400 U/ml) resulted in a strong reduction in binding of anti-C3c and anti-TCC antibodies to the co-cultured beads. This study indicates that proinflammatory cytokines upregulate the synthesis by EC of the functional alternative and terminal pathways of complement.

References

Sep 1, 1992·Immunopharmacology·J P de BoerC E Hack
Jan 1, 1992·European Journal of Immunology·N JulenJ Ripoche
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F CozzolinoD M Stern
Jan 1, 1991·International Archives of Allergy and Applied Immunology·M SchönermarkG M Hänsch
Jun 1, 1991·Scandinavian Journal of Immunology·E Johnson, G Hetland
Aug 18, 1988·The New England Journal of Medicine·E GirardinP H Lambert
May 1, 1988·Scandinavian Journal of Clinical and Laboratory Investigation·E Johnson, G Hetland
Jun 1, 1987·Scandinavian Journal of Immunology·H B PettersenG Hetland
Oct 24, 1986·Science·K J TraceyJ D Albert
Jul 28, 1983·The American Journal of Medicine·W R McCabeA De Maria
Jan 1, 1994·International Journal of Clinical & Laboratory Research·S Perissutti, F Tedesco
Jan 1, 1993·Immunologic Research·A R McPhaden, K Whaley
May 1, 1993·Clinical Immunology and Immunopathology·Y KatzE Lahat
Jul 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·V BergeE Johnson

❮ Previous
Next ❯

Citations

Sep 23, 2000·Brain Research. Molecular Brain Research·R StrohmeyerJ Rogers
Apr 12, 2001·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H LangeggenE Johnson
Jul 11, 2000·Clinical and Experimental Immunology·H LangeggenF Tedesco
Mar 18, 2005·The Journal of Pain : Official Journal of the American Pain Society·Carin M TwiningLinda R Watkins
Jul 13, 2021·Frontiers in Immunology·Alma Yuste-MontalvoVanesa Esteban

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.