Interleukin-1 and interleukin-6 mediated skeletal muscle arteriolar vasodilation: in vitro versus in vivo studies

Shock
A MinghiniM A Hill

Abstract

Interleukin (IL) 1 and IL-6 have been implicated in the decreased systemic vascular resistance of septic shock; however, their sites of action and underlying mechanisms remain unclear. This study determined the effects of IL-1 and IL-6 on rat skeletal muscle arterioles using both in vitro and in vivo preparations. In the in vitro preparation, first order cremasteric arterioles were isolated from rats, cannulated with micropipettes, pressurized to 70 mmHg, superfused with physiologic saline solution, and allowed to achieve spontaneous basal tone in the absence of intraluminal flow. In the in vivo preparation, the cremaster muscle of anesthetized rats was surgically opened, secured as a flat sheet over an optical pedestal, and superfused with physiologic saline solution. Responses of third order arterioles were studied using transillumination video microscopy. In both arteriolar preparations, vessel diameter and phenylephrine (PE) responsiveness were assessed before and after cytokine exposure and washout. In vitro exposure of IL-1 (20 ng/mL (n=8) or 60 ng/mL (n=4)) or IL-6 (500 U/mL (n=2) or 1,000 U/mL (n=4)) for 1 h did not cause arteriolar vasodilation or change in PE responsiveness. However, during a 1 h in vivo exposure of I...Continue Reading

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