Interleukin 1 beta and interleukin 6 repress clofibric acid induction of different P450 isoforms in cultured foetal rat hepatocytes

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
J H ParmentierP Kremers

Abstract

1. Expression of various P450 subfamilies (1A, 2A, 2B, 2C, 3A) have been studied in cultured foetal rat hepatocytes after treatment with clofibric acid, a peroxisome proliferator and prototypic CYP4A inducer in vitro. Ethoxyresorufin O-deethylase activity (EROD, a CYP1A-related activity) as well as 7 alpha-, 16 alpha-, 2 alpha- and 6 beta-testosterone hydroxylase activities (CYP2A, 2B, 2C11 and 3A respectively) were determined during culture. Levels of the corresponding P450 apoproteins were measured by Western blotting. 2. Clofibric acid was able to induce all the P450-dependent activities studied. In most cases this induction required the additional presence of dexamethasone, an agent which promotes differentiation and favours long-term maintenance of the hepatocytes. 3. The major pro-inflammatory cytokines, IL-1 beta and IL-6, decrease the levels of the clofibric acid-induced P450 isoforms, except CYP1A, which was insensitive to IL-6, previous studies having shown that IL-1 beta represses lauric acid 12-hydroxylase activity after induction by clofibric acid. The effects of these cytokines were clearly dose- and time-dependent. The decrease in enzyme activity correlated with a decrease in apoprotein content. 4. The ability of...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 31, 1992·Biochemical and Biophysical Research Communications·C TrautweinM Manns
Feb 1, 1992·The Biochemical Journal·D J Waxman, L Azaroff
Jul 7, 1992·Biochemical Pharmacology·Y L ChenL Chauvelot-Moachon
Jul 1, 1991·American Journal of Obstetrics and Gynecology·W H KuttehB R Carr
Aug 1, 1991·The Journal of Clinical Endocrinology and Metabolism·W H KuttehB R Carr
Jan 1, 1990·Pharmacology & Therapeutics·A B Okey
Dec 1, 1986·Infection and Immunity·P GhezziC A Dinarello
Sep 1, 1987·Cell Differentiation·A BollinneJ E Gielen
Mar 15, 1984·Biochemical and Biophysical Research Communications·F Letawe-GoujonJ E Gielen
Oct 18, 1994·Biochimica Et Biophysica Acta·E T MorganK Wright
Apr 1, 1994·Cell Biology and Toxicology·P KremersJ P Thome
Mar 29, 1994·Biochemical Pharmacology·Y Fukuda, S Sassa

❮ Previous
Next ❯

Citations

Dec 6, 1997·International Journal of Cancer. Journal International Du Cancer·M Gemechu-HatewuP Steinberg
Apr 12, 2001·Clinical and Experimental Dermatology·K J Smith, H G Skelton
Nov 17, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·G W WarrenR A Blouin
Jan 9, 1998·Drug Metabolism Reviews·E T Morgan
Apr 10, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Yifang SunDaniel T Eitzman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.