Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps.

Journal of Applied Oral Science : Revista FOB
Alessandra Cecília Oliveira SilvaBruno Neves Cavalcanti

Abstract

After aggression to the dental pulp, some cells produce cytokines in order to start and control the inflammatory process. Among these cytokines, interleukin-1 beta (IL-1beta) and interleukin-8 (IL-8) emerge as important ones. The purpose of this study was to analyze the location, distribution and concentration of these cytokines in healthy and inflamed dental pulps. Twenty pulps, obtained from healthy third molars (n=10) and from pulpectomies (n=10) were used for the study, with half of each group used for immunohistochemistry and half for protein extraction and ELISA assays. Fibroblasts obtained from healthy dental pulps, stimulated or not by Escherichia coli lipopolysaccharide (LPS), in order to simulate aggression on the cell cultures, were also used and analyzed by ELISA for IL-1beta and IL-8 as complementary information. Data obtained from immunohistochemistry were qualitatively analyzed. Data obtained from ELISA assays (tissue and cells) were statistically treated by the t-test (p<0.05). Immunohistochemically, it was observed that inflamed pulps were strongly stained for both cytokines in inflammatory cells, while healthy pulps were not immunolabeled. ELISA from tissues quantitatively confirmed the higher presence of both...Continue Reading

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Citations

Dec 18, 2012·International Endodontic Journal·M ElsalhyR Raghupathy
Jun 13, 2015·Journal of Oral Science·Yasemin OzdemirCemal Eronat
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Methods Mentioned

BETA
ELISA
protein
Protein Assay

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