PMID: 8594877Dec 1, 1995Paper

Interleukin-1 beta induces interstitial collagenase gene expression and protein secretion in renal mesangial cells

The American Journal of Physiology
D Daphna-Iken, A R Morrison

Abstract

Degradation and tissue remodeling of the extracellular matrix in the normal glomerulus occur through the coordinate action of neutral metalloproteinases, which are in turn regulated by specific inhibitors. Many of these proteins can be secreted by mesangial cells. In the current study, gene regulation of a rat matrix metalloproteinase, interstitial collagenase and its tissue inhibitor of metalloproteinase-1 (TIMP-1), was investigated by Northern blot analysis. Stimulation of rat mesangial cell (RMC) collagenase by interleukin 1 beta (IL-1 beta) produced an increase (> 45-fold) in mRNA which peaked at 12 h. Lesser effects on the TIMP-1 mRNA expression were observed in response to IL-1 beta. Indomethacin did not influence the effect of IL-1 beta on collagenase, and exogenous prostaglandin E2 had no significant effect either on basal or IL-1 beta-stimulated mRNA levels. Collagenase was secreted into the media and showed minimal gelatinolytic activity at 36-h stimulation with IL-1 beta by zymography. By Western immunoblotting, we demonstrated with 24 h of stimulation the secretion of the active form of collagenase, which further increased after 36 h with IL-1 beta compared with the control. When RMC were retreated with genistein an...Continue Reading

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