PMID: 9182730Jun 1, 1997Paper

Interleukin 1-induced calcium signalling in chondrocytes requires focal adhesions

The Biochemical Journal
L LuoC A McCulloch

Abstract

The cytokine interleukin 1 (IL-1) is an important mediator of connective-tissue destruction in arthritic joints but the mechanisms by which IL-1 mediates signal transduction in chondrocytes is poorly understood. Previous results have indicated that IL-1 receptors co-localize with focal adhesions [Qwarnstrom, Page, Gillis and Dower (1988) J. Biol. Chem. 263, 8261-8269], discrete adhesive domains of cells that function in cell attachment and possibly in signal transduction. We have determined whether focal adhesions restrict IL-1-induced Ca2+ signalling in primary cultures of bovine chondrocytes. In cells grown for 24 h on fibronectin, the basal intracellular Ca2+ ion concentration ([Ca2+]i) was 100+/-3 nM. Optimal increases of [Ca2+]i above baseline were induced by 10 nM IL-1 (183+/-30 nM above baseline). There was no significant difference between cells plated on fibronectin or type II collagen (P>0.2; 233+/-90 nM above baseline). Ca2+ transients were significantly decreased by the inclusion of 0.5 mM EGTA in the bathing buffer (74+/-11 nM above baseline), and 1 microM thapsigargin completely blocked Ca2+ transients. Cells plated on poly-(l-lysine) or suspended cells showed no Ca2+ increases, whereas cells grown on fibronectin ...Continue Reading

Citations

Jun 8, 1999·The Journal of Clinical Investigation·R SinghJ R Sedor
Jul 2, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qin WangChristopher A McCulloch
Jan 6, 2009·Immunological Reviews·Mohamed Lamkanfi, Vishva M Dixit
Aug 13, 2013·Periodontology 2000·Yongqiang WangChristopher A McCulloch
Dec 11, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I PitaC F Ide
Oct 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qin WangChristopher A McCulloch
Oct 4, 2006·Nature Reviews. Drug Discovery·Christopher A McCullochHani El-Gabalawy
Nov 26, 2004·The Journal of Biological Chemistry·Qin WangChristopher A McCulloch
Sep 22, 2005·American Journal of Physiology. Endocrinology and Metabolism·Iren KossintsevaB F Mitchell
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qin WangChristopher A McCulloch
Sep 8, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R Tonon, P D'Andrea
Dec 17, 2002·Arthritis and Rheumatism·Rosa Maria BorziAndrea Facchini
Jul 26, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dhaarmini RajshankarChristopher A McCulloch
Mar 14, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Amy L McNultyFarshid Guilak
Jun 30, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Eijiro MaedaTakeo Matsumoto
Oct 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kristiana Kandere-GrzybowskaTheoharis C Theoharides
Mar 3, 2001·Pathophysiology : the Official Journal of the International Society for Pathophysiology·F NishisakaC Hamanishi
Apr 25, 2009·Nitric Oxide : Biology and Chemistry·Dorrin NilforoushanMorris F Manolson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.