PMID: 2788696Jul 1, 1989Paper

Interleukin-1 inhibits thyrotrophin-induced human thyroglobulin gene expression

The Journal of Endocrinology
S YamashitaS Nagataki

Abstract

The regulation of thyroglobulin (Tg) and its specific mRNA by interleukin-1 (IL-1) in cultured human thyrocytes was investigated. Specific binding of 125I-labelled IL-1 on thyrocytes was confirmed by solid-phase binding assay. Thyrocytes dispersed from Graves' thyroid tissues were incubated with TSH with or without recombinant human IL-1. TSH stimulated Tg release from cultured human thyrocytes in a dose- and time-dependent manner. Both IL-1 alpha and beta inhibited TSH-induced Tg release at concentrations ranging from 0.01 to 10 U/ml. The suppressive activities of IL-1 alpha and beta were similar. They did not alter the basal level of Tg release. Unstimulated human thyrocytes did not contain any detectable Tg mRNA, but TSH-stimulated thyrocytes expressed a single species of Tg mRNA (8.5 kb). Both IL-1 alpha and beta inhibited TSH-induced Tg mRNA in a dose-responsive manner. IL-1 (10 U/ml) caused maximal suppression of TSH-induced Tg mRNA to nearly basal levels. In contrast, the gamma-actin mRNA hybridization signal was not altered in control or treated cells. Furthermore, IL-1 stimulated [3H]thymidine uptake into thyrocyte DNA. These results demonstrate that IL-1 directly inhibits TSH-induced Tg gene expression and provide fur...Continue Reading

Citations

Nov 1, 1991·Journal of Cellular Physiology·S B MilamR J Klebe
May 18, 2010·Thyroid : Official Journal of the American Thyroid Association·Y Nagayama
May 15, 2015·The Journal of Endocrinology·Emmely M de VriesAnita Boelen
Jul 9, 2016·Developmental and Comparative Immunology·Edwin J W Geven, Peter H M Klaren
Apr 22, 2017·Endocrine-related Cancer·Theo S PlantingaCarmen E Georgescu
Sep 22, 2017·Journal of Molecular Endocrinology·Marika H TesselaarTheo S Plantinga
Jan 1, 1993·Autoimmunity·A K RasmussenK Bendtzen
Nov 18, 2000·American Journal of Physiology. Cell Physiology·K KasaiY Hattori
Oct 30, 2016·The International Journal of Biological Markers·Kemal BeksacFunda Kosova
Dec 1, 1990·Endocrine Pathology·Berczi Istvan
Feb 10, 2021·Journal of Endocrinological Investigation·L CroceL Chiovato

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