Interleukin-1-mediated H2O2 production by hepatic sinusoidal endothelium in response to B16 melanoma cell adhesion

Journal of Cellular Physiology
M J AnasagastiF Vidal-Vanaclocha

Abstract

We have examined H2O2 production by in vitro enriched hepatic sinusoidal endothelium (HSE) during interleukin-1 beta (IL-1 beta) stimulation and B16 melanoma cell adhesion. Production of H2O2 was quantified by flow cytometry and multiwell plate-scanning fluorimetry of intracellular 2', 7'-dichlorofluorescein (DCFH) oxidation in HSE. Under IL-1 beta treatment there was a 6-fold increase in endothelial cells producing H2O2 (67%) and a 4-fold augmentation in the Kupffer cell population (86%). The average H2O2 content per cell size unit significantly (P < 0.01) increased in endothelial cells (2.6-fold) and Kupffer cells (1.7-fold). In contrast to the homogeneity of Kupffer cells, H2O2 production intensity was largely heterogeneous in IL-1 beta-activated HSE. Enhancement of H2O2 production by IL-beta-treated HSE started at the 4th h and peaked 2-3 h later. The addition of increasing concentration of IL-1 beta to HSE for 4 h caused the progressive activation of H2O2 production by treated cells. The addition of 80 M excess of IL-1 receptor antagonist (IL-1 Ra) 10 min before IL-1 beta treatment abrogated IL-1 beta-mediated enhancement of H2O2. From the 2nd h of B16 melanoma adhesion to HSE there was significantly (P < 0.05) enhancement...Continue Reading

References

Jul 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T W McCloskeyD L Laskin
Nov 1, 1992·Cancer Metastasis Reviews·R LafrenieF W Orr
Nov 1, 1990·Cancer Metastasis Reviews·B U PauliD A Hammer
Mar 1, 1989·Clinical & Experimental Metastasis·L WeissK V Honn
Jan 4, 1989·Journal of the National Cancer Institute·A KöckT A Luger
Oct 1, 1988·The Journal of Clinical Investigation·E DejanaR Giavazzi
Jan 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L WeissK V Honn
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E A Kurt-JonesE R Unanue
Jul 30, 1993·International Journal of Cancer. Journal International Du Cancer·Y NonakaK Orita
May 1, 1993·The Journal of Experimental Medicine·P OroszD N Männel
Apr 1, 1965·Analytical Biochemistry·A S KESTON, R BRANDT
Apr 1, 1965·Analytical Biochemistry·R BRANDT, A S KESTON

❮ Previous
Next ❯

Citations

Mar 25, 2009·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Fernando Vidal-Vanaclocha
May 7, 1997·Journal of the National Cancer Institute·M J AnasagastiF Vidal-Vanaclocha
Aug 25, 2010·Pharmacology & Therapeutics·Gabriella LeonarduzziGiuseppe Poli
Feb 13, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Bård SmedsrødNoriko Yamaguchi
Apr 21, 2011·Journal of Cellular Physiology·Ruei-Zeng LinHwan-You Chang
Mar 28, 2002·The Journal of Toxicological Sciences·Takako HamanoEiji Tanaka
Apr 11, 2003·Journal of Pharmacological Sciences·Masashi NishidaKenji Hamaoka
Jan 13, 2006·Nitric Oxide : Biology and Chemistry·Han-Jung ChaeHyung-Ryong Kim
Feb 24, 2000·The Journal of Pathology·F W OrrD M Nance
May 15, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M J AnasagastiF Vidal-Vanaclocha
Mar 7, 2006·Critical Reviews in Clinical Laboratory Sciences·José M EstrelaElena Obrador

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.