Interleukin-1 β Modulates Melatonin Secretion in Ovine Pineal Gland: Ex Vivo Study

BioMed Research International
A P HermanD Tomaszewska-Zaremba

Abstract

The study was designed to determine the effect of proinflammatory cytokine, interleukin- (IL-) 1β, on melatonin release and expression enzymes essential for this hormone synthesis: arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT) in ovine pineal gland, taking into account the immune status of animals before sacrificing. Ewes were injected by lipopolysaccharide (LPS; 400 ng/kg) or saline, two hours after sunset during short day period (December). Animals were euthanized three hours after the injection. Next, the pineal glands were collected and divided into four explants. The explants were incubated with (1) medium 199 (control explants), (2) norepinephrine (NE; 10 µM), (3) IL-1β (75 pg/mL), or (4) NE + IL-1β. It was found that IL-1β abolished (P < 0.05) NE-induced increase in melatonin release. Treatment with IL-1β also reduced (P < 0.05) expression of AA-NAT enzyme compared to NE-treated explants. There was no effect of NE or IL-1β treatment on gene expression of HIOMT; however, the pineal fragments isolated from LPS-treated animals were characterized by elevated (P < 0.05) expression of HIOMT mRNA and protein compared to the explants from saline-treated ewes. Our study proves that IL-1...Continue Reading

References

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Citations

May 24, 2016·Mediators of Inflammation·A P HermanD Tomaszewska-Zaremba
Nov 11, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Karolina WojtulewiczAndrzej Przemysław Herman
Apr 27, 2019·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Andrii BerbetsOleksandr Yuzko
Feb 5, 2021·Antioxidants·Maria-Angeles Bonmati-Carrion, Antonia Tomas-Loba

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Methods Mentioned

BETA
electrophoresis
PCR
Protein

Software Mentioned

Primer
ImageJ
Rotor
Gene 6000
BestKeeper
GraphPad Prism

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