Interleukin-1 or tumor necrosis factor-alpha augmented the cytotoxic effect of mycobacteria on human fibroblasts: application to evaluation of pathogenesis of clinical isolates of Mycobacterium tuberculosis and M. avium complex

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
Takemasa TakiiKikuo Onozaki

Abstract

Mycobacteria-induced in vitro events reflecting human tuberculosis can contribute to the evaluation of the pathogenesis of Mycobacterium tuberculosis (MTB). In this study, we propose such an in vitro method based on live mycobacteria-induced cytotoxicity to human cell lines. When human lung-derived normal fibroblast cell line MRC-5 was infected with various strains of mycobacteria (M. tuberculosis H(37)Rv and H(37) Ra, Mycobacterium avium 427S and 2151SmO, and Mycobacterium bovis BCG Pasteur and Tokyo), the fibroblasts were killed by mycobacteria according to the degree of virulence. Other human originated macrophage (U-937, THP-1), myeloid (HL-60), and epithelial carcinoma (A549) cell lines exhibited a similar cytotoxic response to virulent mycobacteria. MRC-5 was most susceptible to virulent mycobacteria among various human cell lines examined. The cytotoxicity was enhanced by the proinflammatory cytokines, interleukin-1 (IL-1) and tumor necrosis factor-a (TNF-alpha), which in the absence of mycobacteria stimulate the growth of normal human fibroblasts. This in vitro evaluation system was applied to clinical isolates of drug-sensitive MTB (DS-MTB), drug-resistant MTB (DR-MTB) including multidrug-resistant (MDR-MTB), and M. av...Continue Reading

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Citations

Feb 1, 2013·Science Translational Medicine·Bikul DasAntonio Campos-Neto
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·Takemasa TakiiKikuo Onozaki
Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Takemasa TakiiKikuo Onozaki
Jun 23, 2012·Canadian Journal of Microbiology·Jorge Castro-GarzaFrederick D Quinn

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