PMID: 2095355Dec 21, 1990Paper

Interleukin 1 regulates the expression of osteopontin mRNA by osteoblasts

Molecular and Cellular Endocrinology
C H JinT Suda

Abstract

Osteopontin is a matrix protein which belongs to the integrin superfamily and is involved in cell adhesion. In the present study, we examined the regulation of the mRNA expression of osteopontin by interleukin 1 alpha (IL-1 alpha) in osteoblasts. IL-1 alpha greatly increased the steady-state level of osteopontin mRNA in both a mouse osteoblastic cell line (MC3T3-E1) and mouse primary osteoblast-like cells. The increase in the osteopontin mRNA expression by IL-1 alpha was dose-dependent at a range of 0.004-0.2 nM. This was most likely due to an increase in the transcriptional rate, not to an increase in the stability of osteopontin mRNA. The in vitro nuclear transcription experiment showed that IL-1 alpha-treated MC3T3-E1 cells increased the synthesis of osteopontin mRNA. Besides IL-1 alpha, tumor necrosis factor alpha (TNF-alpha), lipopolysaccharides (LPS) and 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) increased the osteopontin mRNA expression in both the clonal osteoblasts (MC3T3-E1) and the primary osteoblast-like cells. In response to such bone-resorbing agents, primary osteoblast-like cells expressed osteopontin mRNA much more strongly than primary fibroblast-like cells isolated from mouse calvaria. Both IL-1 alpha ...Continue Reading

References

Jan 1, 1990·Annual Review of Nutrition·T SudaN Takahashi
Nov 1, 1988·Endocrinology·N TakahashiT Suda
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A OldbergD Heinegård
Nov 13, 1987·Biochemical and Biophysical Research Communications·K YoonG A Rodan
Jan 1, 1983·Methods in Enzymology·P M Lizardi

❮ Previous
Next ❯

Citations

Aug 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N M RitterW T Butler
Nov 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R A DoddsM Gowen
Aug 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J HoranB D Boyan
Jan 20, 2011·Clinical Oral Investigations·Susanne ProkschAli Al-Ahmad
Apr 21, 1995·Annals of the New York Academy of Sciences·J SodekR H Kim
Apr 21, 1995·Annals of the New York Academy of Sciences·K S Kimbro, R A Saavedra
Feb 11, 2000·Annals of the New York Academy of Sciences·J A EllisonG Z Feuerstein
Mar 14, 2001·American Journal of Respiratory Cell and Molecular Biology·F TakahashiY Fukuchi
Sep 8, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A SethC P Vary
Sep 8, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D M SalterM O Wright
May 21, 2008·The International Journal of Biochemistry & Cell Biology·Kitipong UaesoontrachoonCharles N Pagel
Mar 1, 1997·Osteoarthritis and Cartilage·D MargerieE Bartnik
May 3, 2006·The Journal of Nutritional Biochemistry·Arunabh BhattacharyaGabriel Fernandes
Feb 1, 1994·Kidney International·C M GiachelliR J Johnson
May 1, 1992·Journal of Cellular Biochemistry·T SudaE Abe
Dec 8, 2007·Endocrinology·Mei Hua HongJeffrey N Miner
Apr 19, 2015·Cell Communication and Signaling : CCS·Ichiro MurakamiTadashi Yoshino
Apr 7, 1997·Biochemical and Biophysical Research Communications·T NagasakiS Otani
Jul 29, 2020·Anti-cancer Agents in Medicinal Chemistry·Muhammad A B AshrafArif Malik
Jun 1, 1997·Kidney International·U M MalyankarC M Giachelli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.