Interleukin-1 release by rat synovial cells is dependent on sequential treatment with gamma-interferon and lipopolysaccharide

Arthritis and Rheumatism
W J JohnsonB J Dalton

Abstract

To determine the potential regulatory mechanisms involved in synovial cell interleukin-1 (IL-1) release, the ability of gamma-interferon (gamma-IFN) to influence IL-1 release was assessed. Rat synovial cells cultured in the presence of a variety of stimuli, including lipopolysaccharide (LPS), failed to release IL-1. However, pretreatment of synovial cells with gamma-IFN, followed by LPS stimulation, resulted in increased levels of intracellular IL-1 as well as release of IL-1 from the cell. The level of IL-1 release was dependent on the concentration of both gamma-IFN and LPS, and on length of exposure to the gamma-IFN. The kinetic and dose requirements for gamma-IFN-dependent IL-1 release were similar to those for Ia antigen expression, but LPS was necessary for IL-1 messenger RNA induction, intracellular IL-1 accumulation, and IL-1 release. In addition, sequential treatment, i.e., gamma-IFN followed by LPS, was essential for IL-1 induction. Substitution of phorbol ester or calcium ionophore for gamma-IFN did not result in similar IL-1 release. In addition, induction of IL-1 messenger RNA by another stimulus was not sufficient to result in IL-1 release following LPS treatment. These results suggest that release of IL-1 by rat ...Continue Reading

References

Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J M DayerD R Robinson
Oct 1, 1988·Arthritis and Rheumatism·N J MauritzL Klareskog
Dec 1, 1986·The Journal of Clinical Investigation·P LibbyC A Dinarello
May 1, 1987·The Journal of Experimental Medicine·S J WarnerP Libby
Nov 28, 1985·Journal of Immunological Methods·P L SimonJ C Lee
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P E AuronC A Dinarello
Jan 1, 1981·Methods in Enzymology·B Dalton, K Paucker
Jan 1, 1984·Reviews of Infectious Diseases·C A Dinarello
Jan 1, 1984·Springer Seminars in Immunopathology·J M Dayer, S Demczuk

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Citations

Dec 2, 2009·The Journal of Gene Medicine·Kuniaki HonjoToshikazu Kubo
Nov 21, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shinji TsuchidaToshikazu Kubo
Jan 1, 1991·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Z Davidovitch

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