PMID: 8608158Mar 29, 1996Paper

Interleukin 1 stimulates cholesterol esterification and cholesterol deposition in J774 monocytes-macrophages

Biochimica Et Biophysica Acta
C MazièreJ C Mazière

Abstract

The effects of interleukin 1beta (IL1) in the range of concentration of 10-30 ng/ml on cholesterol metabolism were investigated in the monocyte-macrophage cell line J774. IL1 enhanced cholesterol esterification by [14C]oleic acid and acyl-coenzyme A cholesterol acyl transferase activity in a dose-dependent manner. Incubation of IL1-treated cells with acetylated low density lipoproteins labelled with [3H]cholesteryllinoleate resulted in accumulation of radioactive cholesterol in free and esterified form. Concomitantly, IL1 increased the free and esterified cholesterol intracellular content measured by the cholesterol oxidase technique. The effect of IL1 on cholesterol esterification by oleic acid was not observed in the presence of cycloheximide or of the ACAT inhibitor Sandoz 58 035. IL1 also stimulated cholesterol esterification in other cell types such as human fibroblasts and murine endothelial and smooth muscle cells. The effect of IL1 is specific, since IL2 or tumor necrosis factor (TNF) exhibited no significant activity, whereas oncostatin M only slightly enhanced cholesterol esterification. Since cholesterol deposition is involved in the initiation and progression of the atherosclerotic lesions, these findings highlight ...Continue Reading

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Citations

Apr 12, 2007·American Journal of Physiology. Endocrinology and Metabolism·Mohan R DasuIshwarlal Jialal
Aug 10, 2000·Drugs·P H Chong, B S Bachenheimer
Aug 30, 2002·The Journal of Biological Chemistry·Lisa O'RourkePeter R Shepherd
Apr 9, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·S Devaraj, I Jialal

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