Interleukin-10 attenuates impairment of the blood-brain barrier in a severe acute pancreatitis rat model

Journal of Inflammation
Ronggui LinHeguang Huang

Abstract

Impairment of the blood-brain barrier (BBB) in severe acute pancreatitis (SAP) could result in life-threatening pancreatic encephalopathy. Interleukin-10 (IL-10) is a classical cytokine that is well-known for its strong immunoregulatory and anti-inflammatory abilities. However, whether and how IL-10 protects the BBB in SAP are still unclear. This study includes in vivo experiments using a SAP rat model and in vitro experiments using an in vitro BBB model consisting of a monolayer of brain microvascular endothelial cells (BMECs). The study groups are divided into the control, SAP (in vivo)/TNF-α (in vitro), IL-10 treatment, IL-10 + signal transducer and activator of transcription 3 (STAT3) inhibitor S3I-201 treatment groups. Pancreatic pathological scores, serum amylase, serum TNF-α levels and BBB permeability by Evan's blue assay in SAP rat models were evaluated. BMEC apoptosis in SAP rats or induced by TNF-αin vitro was detected by terminal-deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) and flow cytometry, separately. Expression levels of claudin-5 and proteins involved in the STAT3 signaling pathway were measured by Western blotting. Location and changes of junctional structure of claudin-5 on BMECs were asse...Continue Reading

References

Jan 1, 1992·Annals of Surgery·J SchmidtA L Warshaw
Jun 17, 2004·JAMA : the Journal of the American Medical Association·Vege Santhi SwaroopJonathan E Clain
Jun 21, 2005·Cellular and Molecular Neurobiology·Máiria A DeliMasami Niwa
Aug 23, 2005·Scandinavian Journal of Surgery : SJS : Official Organ for the Finnish Surgical Society and the Scandinavian Surgical Society·M G T RaratyR Sutton
Aug 8, 2006·Current Opinion in Gastroenterology·Stephen J Pandol
Apr 11, 2008·Immunity·John J O'Shea, Peter J Murray
Jul 11, 2008·Immunological Reviews·Anne O'GarraCatherine Hawrylowicz
Oct 22, 2008·Medical Hypotheses·Huanchen ShaRajiv Kumar Jha
Dec 30, 2008·Neurochemistry International·Shinsuke NakagawaMasami Niwa
Jun 2, 2009·Current Opinion in Pharmacology·Shomyseh SanjabiRichard A Flavell
May 24, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Diana LondoñoDiego Cadavid
Jun 7, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Felix DyrnaIngo Bechmann
Aug 24, 2013·PloS One·Mingli LiuJonathan K Stiles
Sep 23, 2014·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Philippe VasseurChristine Silvain
Oct 14, 2014·The American Journal of Pathology·Chen NiZhihai Qin
Nov 30, 2014·Seminars in Cell & Developmental Biology·Reiner F HaseloffIngolf E Blasig
May 8, 2015·Autophagy·Liangkun YouWeidong Han
May 20, 2015·CNS Neuroscience & Therapeutics·Wei-Ye LiuLing Li
Oct 28, 2015·Biochemical and Biophysical Research Communications·Ying ZhangJinlian Zhang
Nov 23, 2016·The Journal of Biological Chemistry·Himakarnika AlluriBinu Tharakan

❮ Previous
Next ❯

Citations

Oct 7, 2019·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Giulia CocoReza Dana
Jul 2, 2020·Archives of Physiology and Biochemistry·Bo ZhaoYunman Li
Jan 27, 2019·Journal of Neuroinflammation·Marta A MałkiewiczPaweł J Winklewski
Jan 9, 2019·Frontiers in Cellular Neuroscience·Yukari Shigemoto-MogamiKaoru Sato
Nov 22, 2018·Brain : a Journal of Neurology·Mélanie Morin-BrureauRichard Miles
May 19, 2019·Cancers·Brennan Olson, Daniel L. Marks
Sep 15, 2020·International Journal of Surgery Case Reports·Yayun XuZiping Zhang
Sep 8, 2021·Experimental & Molecular Medicine·Sherry Sin-Hang YeungRaymond Chuen-Chung Chang

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
flow cytometry

Software Mentioned

ImageJ
SPSS
Graph Pad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Blood Brain Barrier & Cytokines

Some cytokines are able to cross the blood brain barrier through transport systems and enter the cerebrospinal fluid and interstitial fluid spaces. Here is the latest research on cytokines crossing the blood brain barrier and how this can affect tissues within the CNS.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.