Interleukin-10 expression is autoregulated at the transcriptional level in human and murine Kupffer cells

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
P A KnolleG Gerken

Abstract

Interleukin 10 (IL-10) is known to downregulate immune responses. The regulation of IL-10 gene expression therefore determines the outcome of local immune reactions. We investigated time course and downregulation of IL-10 production in primary Kupffer's cells (KC), which are known to secrete IL-10 in response to endotoxin challenge. Human and murine KC were isolated by centrifugal elutriation and investigated for IL-10 gene expression by a two-step amplification procedure (reverse transcriptase-polymerase chain reaction [PCR] followed by T7-polymerase chain reaction). We show that IL-10 messenger ribonucleic acid (mRNA) showed a >450 fold increase in KC 2 hours after endotoxin challenge. IL-10 protein release from KC strictly depended on de novo protein synthesis. Endotoxin mediated increase in IL-10 gene expression was downregulated by exogenous (>350-fold reduction of IL-10 mRNA level), as well as endogenous IL-10 protein, showing a negative autoregulatory feedback loop. IL-10 receptor expression was found to be constitutive and functional in KC. Early expression of IL-10 in KC may be of functional relevance to the outcome of immune and inflammatory reactions in the liver sinusoid. The negative autoregulation of IL-10 express...Continue Reading

References

Mar 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A B LumsdenM H Kutner
Sep 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A M SteffanA Kirn
Oct 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F V Chisari
Apr 1, 1994·European Journal of Immunology·J Péguet-NavarroD Schmitt
Nov 1, 1993·European Journal of Immunology·F PowrieR L Coffman
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A S HoK W Moore
Sep 1, 1993·European Journal of Immunology·F PowrieR L Coffman
Jan 1, 1993·Annual Review of Immunology·K W MooreT R Mosmann
Jul 1, 1996·The Journal of Experimental Medicine·H GrouxM G Roncarolo
Oct 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P A KnolleA W Lohse
Oct 1, 1996·Journal of Clinical Gastroenterology·E HashimotiN Hayashi

❮ Previous
Next ❯

Citations

Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C J Edwards-SmithE E Powell
Oct 26, 2010·Nature Reviews. Immunology·Angus W Thomson, Percy A Knolle
Oct 31, 2002·The European Journal of Neuroscience·Annemarie LedeboerAnne-Marie Van Dam
Aug 20, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Christine Rogez-KreuzPascal Clayette
Oct 7, 2006·Current Opinion in Gastroenterology·S J Karpen, J M Crawford
Jun 17, 1999·Alcoholism, Clinical and Experimental Research·H Tsukamoto
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·D ZaguryR C Gallo
Oct 21, 2015·Liver International : Official Journal of the International Association for the Study of the Liver·Lily DaraNeil Kaplowitz
Jan 31, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Andrea AnnoniMaria Grazia Roncarolo
Apr 20, 2007·Biochimica Et Biophysica Acta·Sònia BenítezJose Luis Sánchez-Quesada
May 30, 2008·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Yong ChenJianping Gong
Aug 21, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Qiang YouCynthia Ju
Jan 28, 2010·Alcoholism, Clinical and Experimental Research·Andrew M MillerBin Gao
Apr 19, 2005·Journal of Neuroimmunology·Sergey G Kremlev, Charles Palmer
Jun 24, 2015·Journal of Hepatology·Yik Chun WongPatrick Bertolino
May 26, 2004·Biochimica Et Biophysica Acta·Christian P SchneiderIrshad H Chaudry
Apr 28, 2009·Toxicologic Pathology·Kazuyoshi KumagaiSunao Manabe
Jan 31, 2018·Nature Immunology·Marc RingelhanMathias Heikenwalder
Mar 25, 2016·World Journal of Transplantation·Felix OldhaferFlorian W R Vondran
Feb 15, 2019·Brain and Behavior·Jin-Yong JoungChang-Gue Son
Nov 8, 2017·Seminars in Immunopathology·Antonella Carambia, Johannes Herkel
Jul 10, 2002·Current Opinion in Nephrology and Hypertension·Mariusz L KielarChristopher Y Lu
Jan 18, 2020·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Timucin TanerMark D Stegall
Mar 21, 2017·Digestive Diseases and Sciences·Nathaniel Aviv Cohen, Nitsan Maharshak
May 10, 2017·International Journal of Molecular Sciences·Andrea IorgaNeil Kaplowitz
Oct 16, 2019·Frontiers in Immunology·Karoline KielbassaLeila Akkari
May 23, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Philippe MathurinHidekazu Tsukamoto
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marc RiemannRoland M Schmid
Jan 17, 2017·Clinical & Translational Immunology·Dirk Wohlleber, Percy A Knolle
Nov 27, 2018·Frontiers in Immunology·Hongting HuangQiang Xia
May 10, 2019·Frontiers in Immunology·Chris John WestonDavid H Adams
May 24, 2019·In Vitro Cellular & Developmental Biology. Animal·Yu ShimizuAkimasa Fukui
Sep 8, 2001·American Journal of Physiology. Cell Physiology·M G SchwachaI H Chaudry
Mar 16, 2017·Pflügers Archiv : European journal of physiology·Zeinab Abdullah, Percy A Knolle
May 10, 2019·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Julia KhantakovaSergey Sennikov
May 5, 2020·Frontiers in Immunology·Zhao Shan, Cynthia Ju
Jul 1, 2020·Frontiers in Immunology·Yanzhi JiangXiao-Kang Li
May 16, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Annemarie LedeboerAnne-Marie Van Dam

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.