Interleukin-10 inhibits proinflammatory chemokine release by neutrophils of the newborn without suppression of nuclear factor-kappa B

Pediatric Research
Johny TryzmelDennis Davidson

Abstract

An increase in polymorphonuclear leukocytes (PMNs) and proinflammatory chemokines, such as IL-8 and macrophage inflammatory protein-1 alpha (MIP), are found in the airways during early stages of bronchopulmonary dysplasia. We determined whether IL-10 produces a dose-related inhibition of proinflammatory chemokine release from stimulated neutrophils of the newborn and whether the mechanism involves the pivotal transcription factor, nuclear factor-kappa B. PMNs isolated from the cord blood of healthy newborns were stimulated submaximally with either lipopolysaccharide (n = 5) or tumor necrosis factor (n = 4), with and without IL-10 (0.01-1000 ng/mL). IL-8 and MIP release were measured in cell culture supernatants at 18 h. The presence or absence of nuclear factor-kappa B activity and inhibitor-kappa B alpha degradation was measured at 30 min and 3 h after PMN stimulation began. During lipopolysaccharide stimulation, IL-10 significantly reduced IL-8 levels from 50 +/- 16 ng/mL to 7 +/- 3 ng/mL, and MIP levels from 14 +/- 5 to 0.7 +/- 0.1 ng/mL (mean +/- SEM, p < 0.01). IL-10 produced an insignificant reduction in IL-8 and MIP levels after stimulation of PMNs with tumor necrosis factor. IL-10 did not inhibit nuclear factor-kappa B ...Continue Reading

References

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Citations

Jul 21, 2004·Biology of the Neonate·Nazeeh HannaBarry Weinberger
Jan 18, 2013·Fibrogenesis & Tissue Repair·Anu S MaharjanRichard H Gomer
Feb 5, 2009·Experimental Lung Research·Purnima JoseDonald A Cohen
Dec 30, 2014·Cytokine·Benoît LevastJoaquín Madrenas
Dec 21, 2007·The Korean journal of laboratory medicine·Ji Weon SeoHan Ik Cho
Dec 11, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hyeon-Soo LeeJuan Sanchez-Esteban
May 9, 2014·Journal of Leukocyte Biology·Nehemiah CoxRichard H Gomer

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