PMID: 9178819May 16, 1997Paper

Interleukin-10 is a growth factor for human melanoma cells and down-regulates HLA class-I, HLA class-II and ICAM-1 molecules

International Journal of Cancer. Journal International Du Cancer
F Y YueG Burg

Abstract

IL-10 is a cytokine which shows various effects including inhibition of T-cell proliferation or HLA-dependent antigen presentation. In this study, we analysed the effects of exogenous or autocrine IL-10 on proliferation and expression of immunocritical surface molecules. Fourteen cultures of human melanoma cells were established from primary melanomas, locoregional lymph-node or distant metastases. In 5 melanoma cell cultures, proliferation in the presence of IL-10, anti-IL-10 antibodies (Ab) or control Ab was assessed with colorimetric and [3H]thymidine uptake assays. Flow cytometric analysis was used to quantify the expression of human leukocyte antigen (HLA) class-I, HLA class-II and intercellular adhesion molecule (ICAM)-1 and the IL-10 receptor (IL-10R). IL-10 production of melanoma cells was documented by RT-PCR and IL-10 protein was detected in the supernatants by means of ELISA. IL-10 enhanced proliferation and prolonged survival of melanoma cells in 5 out of 5 cultures. Anti-IL-10 Ab decreased proliferation. IL-10R expression was found in 12 out of 14 (86%) melanoma cell cultures. The expression of HLA-I, HLA-II and ICAM-1 on all melanoma cells that were positive for IL-10R showed a reduction of 10-60% by IL-10, wherea...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P PisaR Kiessling
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P VieiraK W Moore
Dec 1, 1989·The Journal of Experimental Medicine·D F FiorentinoT R Mosmann
Jul 1, 1995·British Medical Bulletin·A SlominskiM C Mihm
Sep 1, 1995·Cancer Immunology, Immunotherapy : CII·E C LattimeD Berd
Dec 1, 1994·British Journal of Cancer·S Krüger-KrasagakesT Diamantstein
Mar 1, 1994·International Journal of Cancer. Journal International Du Cancer·Q ChenP Hersey
Aug 19, 1993·International Journal of Cancer. Journal International Du Cancer·G A GastlN H Bander
May 29, 1996·International Journal of Cancer. Journal International Du Cancer·W DummerE B Bröcker

❮ Previous
Next ❯

Citations

Oct 31, 2002·Nature Immunology·Hung T Khong, Nicholas P Restifo
Jan 5, 2002·Journal of Virology·Juliet V SpencerThomas J Schall
Feb 24, 2004·Infection and Immunity·Jennifer A ShumillaElizabeth D Mellins
Dec 28, 2007·Experimental & Molecular Medicine·Chien-Fu HungT-C Wu
Aug 14, 2008·World Journal of Gastroenterology : WJG·Giacomo LanzoniGian Paolo Bagnara
Mar 5, 2010·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Jun-Han SuT-C Wu
Aug 4, 2010·Archivum Immunologiae Et Therapiae Experimentalis·Akshata Udyavar, Terrence L Geiger
Jun 8, 2012·Cancer Immunology, Immunotherapy : CII·Miriam AlbDavid Schrama
Nov 8, 2011·Immunotherapy·Judith LeiboviciJudith Sinai
Oct 28, 2015·Expert Review of Clinical Immunology·Francesco MannavolaFranco Silvestris
Mar 21, 2008·Expert Opinion on Biological Therapy·Chien-Fu HungT-C Wu
Dec 1, 2001·Expert Opinion on Biological Therapy·G Q PhanF M Marincola
Feb 3, 2016·Human Vaccines & Immunotherapeutics·Joseph G SkeateW Martin Kast
Feb 4, 2010·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Ken LinT C Wu
Apr 24, 2013·Clinics in Dermatology·Julien Fourcade, Hassane M Zarour
Dec 1, 2010·Cytokine & Growth Factor Reviews·Robert SabatJens Geginat
Mar 5, 2002·The Journal of Investigative Dermatology·Robert A OlieUwe Zangemeister-Wittke
Oct 28, 2008·Burns : Journal of the International Society for Burn Injuries·Yoshitaka MatsumotoNoriyuki Sato
Feb 24, 2016·Expert Review of Vaccines·Andrew YangChien-Fu Hung
Nov 6, 2007·Seminars in Cancer Biology·Mirjana Urosevic
Jun 19, 2007·Cancer Genetics and Cytogenetics·Meri-Sisko Vuoristo
Jul 6, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S TrivediR L Ferris
Mar 3, 2007·Cytokine & Growth Factor Reviews·Flavio Salazar-OnfrayAriadna Mendoza-Naranjo
Feb 20, 2007·Molecular Aspects of Medicine·Massimo MasieroElena Favaro
Jan 6, 2012·Journal of Oncology·E Guzmán-OleaV Madrid-Marina
Nov 18, 2004·Immunological Reviews·Alain P Vicari, Giorgio Trinchieri
Sep 17, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Hearn Jay Cho, Nina Bhardwaj
Jul 23, 2008·Experimental Dermatology·Dan Ilkovitch, Diana M Lopez
Mar 28, 2008·Immunological Reviews·Chien-Fu HungRichard Roden
Jul 11, 2008·Immunological Reviews·Anne O'GarraCatherine Hawrylowicz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.