Interleukin-12 activates human gamma delta T cells: synergistic effect of tumor necrosis factor-alpha

European Journal of Immunology
C UetaI Tsuyuguchi

Abstract

gammadelta T cell populations are known to expand in response to intracellular bacterial infectious agents regardless of previous priming. We have shown previously that soluble factor(s) produced by Mycobacterium-stimulated monocytes activate cord blood gammadelta T cells to proliferate. In this study, we investigated whether cytokines produced by monocytes are responsible for gammadelta T cell activation in vitro: interleukin (IL)-1beta, IL-6, IL-8, IL-12, tumor necrosis factor (TNF)-alpha and granulocyte/macrophage colony-stimulating factor were examined. Recombinant human IL-12 stimulated gammadelta T cells, but not alphabeta T cells in peripheral blood mononuclear cells, to express CD25 on their surfaces, and to expand in number in vitro. IL-12-primed gammadelta T cell numbers increased to a greater extent in the culture to which exogenous IL-2 (5 U/ml) was added. Anti-TNF-alpha monoclonal antibody inhibited IL-12-induced up-regulation of CD25 on gammadelta T cells, suggesting that endogenous TNF-alpha may play a role in IL-12-induced activation of gammadelta T cells. Recombinant TNF-alpha synergistically augmented IL-12-induced activation of gammadelta T cells. Furthermore, IL-12 up-regulated TNF receptors on gammadelta T ...Continue Reading

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Citations

Oct 18, 2002·Veterinary Immunology and Immunopathology·J M Pollock, M D Welsh
Jan 25, 2003·Burns : Journal of the International Society for Burn Injuries·Martin G Schwacha
May 9, 2000·Microbes and Infection·D Kabelitz
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Feb 13, 2015·Journal of Virology·Marco CardoneLucia Conti
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Sep 19, 2015·International Journal of Cancer. Journal International Du Cancer·Kilian Wistuba-HamprechtGraham Pawelec

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