PMID: 9421853Jan 9, 1998Paper

Interleukin-13 is a more potent inhibitor of the expression of inducible nitric oxide synthase in smooth muscle cells than in macrophages: a comparison with interleukin-4 and interleukin-10

Shock
H Ruetten, C Thiemermann

Abstract

This study compares the effects of interleukin (IL)-13, a cytokine with anti-inflammatory properties, with those of IL-4 or IL-10 on the expression of inducible nitric oxide synthase (iNOS) protein and activity in 1) a murine macrophage cell line (J774.2) activated with lipopolysaccharide (LPS) and 2) rat aortic smooth muscle cells (RASM) activated with LPS plus interferon-gamma. Pretreatment of macrophages with IL-4 or IL-13 caused a similar, concentration-dependent inhibition of the formation of nitrite and the expression of iNOS protein elicited by LPS. In contrast, IL-13 was a much more potent inhibitor of the formation of nitrite and the expression of iNOS protein in activated RASM than IL-4. IL-10 caused only a small, but significant, inhibition of the nitrite formation induced by LPS in macrophages and RASM. Pretreatment of J774.2 macrophages, but not of RASM, with the phosphatidylinositol-3-kinase inhibitor, wortmannin (10-100 nM), attenuated the inhibition by either IL-13 or IL-4 of the LPS-induced increase in nitrite in a dose-related fashion. Thus, IL-13 is more potent than IL-4 in preventing the expression of iNOS protein and activity in activated RASM, whereas IL-13 and IL-4 are equipotent in inhibiting the express...Continue Reading

Citations

Apr 14, 2005·Cytokine·A B Paoliello-PaschoalatoF Q Cunha
Mar 26, 1999·European Journal of Biochemistry·M Díaz-CazorlaS Lamas
May 15, 2002·Glia·Myung-Soon YangEun-Hye Joe
Aug 29, 2007·Biochemical and Biophysical Research Communications·Lifang ShaoDavid A Geller
Aug 5, 2008·American Journal of Obstetrics and Gynecology·Hyagriv N SimhanMarijane A Krohn
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Mar 14, 2003·Cancer Cell International·JiYan ZhangBeiFen Shen
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