Interleukin-13 is produced by activated human CD45RA+ and CD45RO+ T cells: modulation by interleukin-4 and interleukin-12

European Journal of Immunology
T JungH Yssel

Abstract

Interleukin (IL)-13 is a cytokine originally identified as a product of activated T cells. Little is known however, about IL-13 production by human T cells and its modulation by other cytokines. Here, we show that IL-13 is produced by activated human CD4+ and CD8+ CD45RO+ memory T cells and CD4+ and CD8+ CD45RA+ naive T cells. In contrast, IL-4, which shares many biological activities with IL-13, is only produced by CD45RO+ T cells following activation. Analysis of intracellular cytokine production by single CD45RA+ and CD45RO+ T cells indicated that IL-13 continued to be produced for more than 24 h after stimulation, whereas IL-4 could not be detected after 24 h. These data were confirmed by measurement of specific mRNA and suggest that IL-13, unlike IL-4, but like interferon-gamma (IFN-gamma), is a cytokine with long-lasting kinetics. The majority of human CD45RO+ T cells produced IL-4 and IL-13 simultaneously. In contrast, IFN-gamma protein was generally not co-expressed with IL-4 or IL-13. IL-4 added to primary cultures of highly purified peripheral blood T cells activated by the combination of anti-CD3+anti-CD28 mAb enhanced IL-13 production by CD45RA+ and to a lesser extent by CD45RO+ T cells. Under these conditions, howe...Continue Reading

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