Interleukin-15 enhances cytotoxicity, receptor expression, and expansion of neonatal natural killer cells in long-term culture

Clinical and Diagnostic Laboratory Immunology
Sunwoong S ChoiRobert L Roberts

Abstract

Newborn infants have a higher susceptibility to various pathogens due to developmental defects in their host defense system, including deficient natural killer (NK) cell function. In this study, the effects of interleukin-15 (IL-15) on neonatal NK cells was examined for up to 12 weeks in culture. The cytotoxicity of fresh neonatal mononuclear cells (MNC) as assayed by K562 cell killing is initially much less than that of adult MNC but increases more than eightfold after 2 weeks of culture with IL-15 to a level equivalent to that of adult cells. This high level of cytotoxicity was maintained for up to 12 weeks. In antibody-dependent cellular cytotoxicity (ADCC) assays using CEM cells coated with human immunodeficiency virus gp120 antigen, IL-15 greatly increased ADCC lysis by MNC from cord blood. IL-15 increased expression of the CD16+ CD56+ NK markers of cord MNC fivefold after 5 weeks of incubation. Cultures of neonatal MNC with IL-15 for up to 10 weeks resulted in a unique population of CD3- CD8+ CD56+ cells (more than 60%), which are not present in fresh cord MNC. These results show that IL-15 can stimulate neonatal NK cells and sustain their function for several weeks, which has implications for the clinical use of IL-15.

References

May 28, 1981·Nature·C S HenneyS Gillis
May 1, 1994·Clinical and Diagnostic Laboratory Immunology·E R StiehmM W Fanger
Aug 1, 1995·Endocrinology·L S QuinnK H Grabstein
Jun 1, 1995·Clinical and Experimental Immunology·Y OsugiA Tawa
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·M ClericiP A Henkart
Dec 15, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·H FarzadeganJ B Margolick
Mar 17, 2001·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·S J LinD C Yan

❮ Previous
Next ❯

Citations

Apr 16, 2011·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Yu ZhaoJi-Yan Liu
Apr 23, 2013·Clinical & Developmental Immunology·Soren Gantt, William J Muller
Sep 26, 2013·Clinical & Developmental Immunology·Yen-Chang Lee, Syh-Jae Lin
Jan 19, 2016·Frontiers in Medicine·Rohtesh S MehtaKatayoun Rezvani
Oct 3, 2009·British Journal of Haematology·Michael R Verneris, Jeffrey S Miller
Mar 22, 2015·Cytotherapy·Jeannette CanyNina Shah
Oct 6, 2011·Pediatric Hematology and Oncology·Syh-Jae Lin, Ming-Ling Kuo
Jan 16, 2015·Journal of Pediatric Hematology/oncology·Kimberly A McDowellPaul M Sondel
Oct 9, 2016·Cytometry. Part B, Clinical Cytometry·Dara M Strauss-AlbeeCatherine A Blish
Dec 10, 2008·International Reviews of Immunology·Syh-Jae LinMing-Ling Kuo
Jun 23, 2018·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Tamara TyrinovaElena Chernykh
Dec 2, 2020·International Reviews of Immunology·Elaheh ArianfarAli Memarian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Adrenocortical carcinoma (ACC) is a rare malignancy of the adrenal cortex, associated with a generally dismal prognosis owing to its aggressive behavior. Here are the latest discoveries pertaining to this disease.

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.