Interleukin-1beta induced changes in the protein expression of rat islets: a computerized database

Electrophoresis
H U AndersenJ Nerup

Abstract

Insulin-dependent diabetes mellitus is caused by an autoimmune destruction of the beta-cells in the islets of Langerhans. The cytokine interleukin 1 inhibits insulin release and is selectively cytotoxic to beta-cells in isolated pancreatic rat islets. The antigen(s) triggering the immune response as well as the intracellular mechanisms of action of interleukin 1-mediated beta-cell cytotoxicity are unknown. However, previous studies have found an association of beta-cell destruction with alterations in protein synthesis. Thus, two-dimensional (2-D) gel electrophoresis of pancreatic islet proteins may be an important tool facilitating studies of the molecular pathogenesis of insulin-dependent diabetes mellitus. 2-D gel electrophoresis of islet proteins may lead to (i) the determination of qualitative and quantitative changes in specific islet proteins induced by cytokines, (ii) the determination of the effects of agents modulating cytokine action, and (iii) the identification of primary islet protein antigen(s) initiating the immune destruction of the beta-cells. Therefore, the aim of this study was to create databases (DB) of all reproducibly detectable protein spots on 10% and 15% acrylamide 2-D gels of neonatal rat islets (10%...Continue Reading

References

Apr 1, 1990·Immunology Today·S H Kaufmann
Jan 1, 1994·Molecular Reproduction and Development·C Z ShiS Heyner
Oct 1, 1995·Electrophoresis·S SteinerP Prieto
Sep 1, 1996·Diabetologia·T Mandrup-Poulsen

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Citations

Oct 13, 2009·Diabetes, Obesity & Metabolism·P Bergsten
Jul 13, 2002·American Journal of Medical Genetics·Flemming Pociot, Allan E Karlsen
Aug 9, 2011·Expert Review of Proteomics·Jian-Ying ZhouWei-Jun Qian
Dec 22, 2006·Expert Review of Proteomics·Henrik Ortsäter, Peter Bergsten
Apr 13, 2006·Expert Review of Proteomics·Wannes D'HertogLut Overbergh
Sep 28, 2010·Archives of Physiology and Biochemistry·James N McGuireFlemming Pociot
May 1, 2008·Proteomics. Clinical Applications·Svante ResjöOla Hansson
Apr 8, 2006·Biochemical and Biophysical Research Communications·Allan E KarlsenFlemming Pociot
Feb 3, 2015·Proteomics. Clinical Applications·Inne CrèvecoeurLut Overbergh
Feb 9, 2005·Molecular & Cellular Proteomics : MCP·Thomas SparreFlemming Pociot
Jul 6, 2017·Critical Reviews in Food Science and Nutrition·Shubhra PandeAlla B Salmina
Nov 25, 2003·Expert Review of Molecular Diagnostics·Thomas SparreFlemming Pociot
Mar 5, 2013·Proteomics·MeiHwa Tanielle Bench AlvarezSteven W Graves
Jul 1, 2006·Expert Review of Endocrinology & Metabolism·Visith Thongboonkerd

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