Interleukin-1beta-induced expression of IL-6 and production of human chorionic gonadotropin in human trophoblast cells via nuclear factor-kappaB activation

American Journal of Reproductive Immunology : AJRI
Satoru TsukiharaNaoki Terakawa

Abstract

The cytokine, interleukin-6 (IL-6), stimulates the production of human chorionic gonadotropine (hCG) in chorionic cells. The purpose of this study was to examine the role of nuclear factor-kappaB (NF-kappaB) during the induction of IL-6 by IL-1beta in human trophoblast cells. Reverse transcription-polymerase chain reaction was used to determine the gene expressions of IL-1, IL-1 receptor (IL-1R), IL-6R and gp130 in a human choriocarcinoma cell line, BeWo. The BeWo cells were cultured for 24 hr with IL-1beta (0-10 ng/mL), IL-1beta (10 ng/mL) together with IL-1 receptor antagonist (IL-1Ra) or NF-kappaB inhibitor, N-tocyl-l-phenylalanine chloromethyl ketone (TPCK). The concentrations of IL-6 in culture supernatants were measured by enzyme-linked immunosorbent assay. The gene expressions of IL-1R, IL-6R and gp130, but not IL-1beta, were observed. The concentration of IL-6 was enhanced by IL-1beta in a dose-dependent fashion. The addition of IL-1Ra neutralized the effects of IL-1beta on IL-6 secretion. IL-1beta induced expression of phosphorylated IkappaB and the activation of NF-kappaB. The addition of TPCK reduced the IL-1beta-induced IL-6 expression. Adding IL-1beta increased beta-hCG production in a dose-dependent manner, and th...Continue Reading

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Citations

Jun 3, 2014·Journal of Translational Medicine·Letícia S Castro-FiliceEloisa A V Ferro
Apr 7, 2009·Life Sciences·Tatsuya FukamiTatsuhiko Kawarabayashi
Feb 15, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Alice MeunierMichel Pohl
Feb 14, 2009·The Journal of Pathology·Yuan LiuZhigang Zhang
Nov 28, 2006·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Carlo TicconiCh V Rao
Jun 15, 2007·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Ysabel C CasartMaría I Camejo
Jun 1, 2011·American Journal of Reproductive Immunology : AJRI·Michail KalinderisBasil C Tarlatzis
May 16, 2021·Cell Death & Disease·Stefan TeufelChristine Hartmann

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