Interleukin-1beta induces the expression of lipocortin 1 mRNA in cultured rat cortical astrocytes

Neuroscience Research
T MiyachiT Kato

Abstract

Lipocortin 1 (LC1) has been shown to increase in neuronal damage and act as a neuroprotectant and a neurotrophic factor. IL-1beta acts as a mediator of inflammation and has been reported as a potent inducer of various neurotrophic factors including nerve growth factor and fibroblast growth factor. In this study, we investigated the relationship between LC1 and IL-1beta in cultured rat astrocytes. Time-and dose-dependent experiments of IL-1beta on rat cortical astrocytes in culture revealed that the expression of LC1 mRNA was significantly augmented by IL-1beta at 8 h, 10 ng/ml. In addition, IL-1beta evoked an extracellular secretion of LC1 without its cytotoxic effects. The effect of IL-1beta was completely abolished when we treated cells with inhibitor of mitogen-activated protein kinases (MAPKs) (PD98059) (25 microM), phospholipase A(2) inhibitor mepacrine (30 microM) and protein synthesis inhibitor cycloheximide (CHX) (10 microg/ml). This suggests that induction of LC1 by IL-1beta is through a MAPKs and phospholipaseA(2) pathway and requires protein synthesis. These results indicate that IL-1beta released in the central nervous system (CNS) injury can stimulate the transcription of the LC1 gene. Subsequent synthesis and rele...Continue Reading

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Citations

Mar 3, 2007·Der Orthopäde·O I SchmidtP F Stahel
Jan 17, 2013·Cellular and Molecular Neurobiology·Hayato AsaiKiyofumi Asai
Mar 28, 2008·Science in China. Series C, Life Sciences·LingLing Hou, Tao Hong
Apr 15, 2004·Molecular and Cellular Neurosciences·Raghuram SelvarajuUrsula Boschert
Apr 6, 2004·Progress in Neurobiology·Kazuhiro TakumaToshio Matsuda
Aug 21, 2012·Journal of Neuroscience Research·M AoyamaK Asai
Dec 31, 2008·Journal of Cellular Physiology·Silvia Di LoretoFernanda Amicarelli
Oct 1, 2013·Reviews in the Neurosciences·Francisco Javier Medina, Isaac Túnez
Sep 11, 2019·Journal of Neuropathology and Experimental Neurology·Masahiro ShijoToru Iwaki
Dec 10, 2020·Neural Plasticity·Gabriella PolicastroGiovanna Paolone
Sep 11, 2021·Behavioural Brain Research·Nasser RahmaniJalal Zaringhalam
Jul 7, 2009·Neuroscience Letters·Kentaro OharaKiyofumi Asai

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