Interleukin-1beta inhibits growth factor-stimulated restoration of wounded rat gastric epithelial cell monolayers

Digestive Diseases and Sciences
E NakamuraS Okabe

Abstract

We examined the effect of interleukin-1beta (IL-1beta) on spontaneous and enhanced restoration (cell migration and proliferation) using an in vitro wound model comprising a confluent monolayer of rat gastric epithelial RGM1 cells. Repair of an artificial wound in a cell monolayer was found to be time- and concentration-dependent when the cells were incubated with epidermal growth factor (EGF) or transforming growth factor (TGF) -alpha alone for up to 24 hr. The growth factors also stimulated DNA synthesis significantly for 24 hr in a concentration-related manner. IL-1beta had no effect on wound restoration in the absence of the growth factors. However, it markedly inhibited the restoration enhanced by EGF and TGF-alpha, the inhibition being about 60% and 70%, respectively. In addition, IL-1beta significantly reduced the DNA synthesis stimulated by the growth factors. The EGF- and TGF-alpha-enhanced restoration was reduced by about 30% by mitomycin C, which potently inhibited the stimulated DNA synthesis. Mitomycin C had no effect on the spontaneous restoration. Even when treated with mitomycin C, the inhibitory effect of IL-1beta on the enhanced wound repair was still observed; however, the extent of the inhibition was decrease...Continue Reading

Citations

Oct 13, 2006·Current Opinion in Gastroenterology·P Holzer
Dec 14, 2001·Diseases of the Colon and Rectum·K R ShaperP B Boulos
Feb 23, 2005·In Vitro Cellular & Developmental Biology. Animal·Miguel R Reyes, Brissia Lazalde
Dec 27, 2016·Journal of Cellular Physiology·Anja Paehler Vor der NolteUrsula Seidler
Dec 29, 2020·Journal of Ethnopharmacology·Gisele Goulart da SilvaAna Lucia Tasca Gois Ruiz

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