Interleukin-1beta secreted from monocytic cells induces the expression of matrilysin in the prostatic cell line LNCaP.

The Journal of Biological Chemistry
R D KleinG T Bowden

Abstract

Matrilysin is a matrix metalloprotease that is overexpressed in cancer cells of epithelial origin and in normal tissues during events involving matrix remodeling such as the cycling endometrium. We previously observed that inflamed ductule and acinar epithelia in the prostate also overexpress matrilysin. The presence of infiltrating macrophages in these areas prompted us to determine if factors secreted from monocytes could induce matrilysin expression in a human prostatic cell line. Conditioned media collected from the monocyte cell line THP-1 following lipopolysaccharide treatment substantially induced matrilysin protein and mRNA expression in LNCaP prostate carcinoma cells. Matrilysin expression in LNCaP cells was also induced by recombinant interleukin (IL)-1 (50 pM), but not by equimolar concentrations of recombinant tumor necrosis factor-alpha or IL-6. The matrilysin-inducing activity of THP-1 conditioned medium was completely abrogated by preincubation with a neutralizing antibody to IL-1beta. Transient transfection analyses with a chimeric human matrilysin promoter-chloramphenicol acetyltransferase reporter construct demonstrated that IL-1beta activates transcription through the matrilysin promoter in LNCaP cells. This ...Continue Reading

References

Jun 19, 1991·International Journal of Cancer. Journal International Du Cancer·D MullerJ Abecassis
Jan 1, 1991·Journal of Cancer Research and Clinical Oncology·M S PajouhG T Bowden
Dec 1, 1989·Arthritis and Rheumatism·S S McCachrenJ E Niedel
Jul 1, 1988·Archives of Biochemistry and Biophysics·C W LinC H Evans
Oct 31, 1988·Biochemical and Biophysical Research Communications·M TurnerM Feldmann
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S B MizelS E Mergenhagen
Aug 1, 1980·International Journal of Cancer. Journal International Du Cancer·S TsuchiyaK Tada
Nov 15, 1993·Biochemical and Biophysical Research Communications·P G Mitchell, H S Cheung
May 1, 1994·The Journal of Urology·M J SiegsmundI Pastan
Aug 1, 1995·The Journal of Investigative Dermatology·U K Saarialho-KereW C Parks
Apr 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·M Z JasserH S Cheung
Oct 1, 1994·The Journal of Investigative Dermatology·T V KarelinaA Z Eisen
Dec 1, 1993·Journal of Cellular Biochemistry·A Mauviel
Jan 1, 1993·American Journal of Obstetrics and Gynecology·W H RodgersF Gorstein
Jan 1, 1996·Molecular Carcinogenesis·J D KnoxR B Nagle
Feb 1, 1996·The International Journal of Biochemistry & Cell Biology·C L Wilson, L M Matrisian
Sep 1, 1996·The Prostate·W C PowellG T Bowden

❮ Previous
Next ❯

Citations

Jun 4, 1999·Trends in Cell Biology·M Colledge, J D Scott
Oct 14, 1998·The Journal of Clinical Investigation·S E DunsmoreW C Parks
Oct 12, 2010·Digestive Diseases and Sciences·Xia WangXianfei Wang
Mar 22, 2000·The Journal of Cell Biology·Y S López-BoadoW C Parks
Jun 25, 2013·Cellular & Molecular Biology Letters·Sudhakar Veeranki
Feb 24, 2015·Digestive Diseases and Sciences·Doron YablecovitchFred M Konikoff
Apr 13, 2018·International Journal of Molecular Sciences·Chiang-Ting WangTsung-Ming Chen
Jul 9, 2020·Cancers·Cédric Rébé, François Ghiringhelli
Jan 5, 2002·Neoplasia : an International Journal for Oncology Research·M S Maliner-StrattonG T Bowden

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
Berolla SahdoBo Söderquist
Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
M Abolhassani, J W Chiao
© 2021 Meta ULC. All rights reserved