PMID: 6980447Feb 1, 1982Paper

Interleukin-2 and serum thymic factor enable autologous rosette-forming T lymphocytes to generate helper and cytotoxic functions

Scandinavian Journal of Immunology
I Sugawara, R Palacios

Abstract

The autologous rosette-forming T cells (Tar cells) isolated by means of their ability to form rosettes with autologous erythrocytes were characterized by the use of OKT monoclonal anti-human T-cell subset antibodies and a monoclonal anti-HLA-DR antibody. We found that the phenotype of Tar cells was OKT 3+4+8+Dr- as determined by both indirect immunofluorescence microscopy and complement-mediated killing of 51Cr-labelled Tar cells. In addition, we found that Tar lymphocytes were able to develop cytotoxicity against allogeneic and trinitrophenol (TNP)-conjugated autologous target cells in the presence of interleukin-2 (IL-2) or serum thymic factor. However, these cells showed little or nor cytotoxicity in the absence of interleukin-2 or serum thymic factor. Tar lymphocytes generated helper function for B lymphocytes in the presence of interleukin-2 in both pokeweed mitogen (PWM)- and purified protein derivative (PPD)-stimulated cultures. Nevertheless, non-IL-2-treated Tar cells did not exhibit any helper activity on B cells. Finally, pretreatment of Tar cells with 1000-1500 rad of X ray made these cells unable to develop helper function for B lymphocytes. It is concluded that: (1) OKT 3+4+8+Dr- Tar cells are able to generate cyto...Continue Reading

References

Aug 1, 1976·European Journal of Immunology·E GronowiczF Melchers
Aug 1, 1974·European Journal of Immunology·G M Shearer
Sep 1, 1981·Scandinavian Journal of Immunology·R Palacios
Jun 1, 1980·The Journal of Clinical Investigation·R PalaciosE Díaz-Jouanen
May 1, 1981·The Journal of Experimental Medicine·R Palacios, G Möller
Apr 30, 1981·Nature·R Palacios, G Möller
Apr 1, 1980·Cell·E L Reinherz, S F Schlossman

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