Interleukin-2 binds to ganglioside GD(1b)

Biochemical and Biophysical Research Communications
M H RavindranathS O'Day

Abstract

We have developed a solid matrix immunoassay to determine the binding of interleukin-2 (IL-2) to specific gangliosides. The assay establishes that recombinant human IL-2 binds to ganglioside GD(1b) but not to any other gangliosides (GM(1), GM(2), GM(3), GD(1a), GD(2), GD(3), and GT(1b)). The binding varies with the ratio of GD1b and IL-2. This assay enables distinguishing the nature of the sugar moiety of the ganglioside recognized by IL-2 and establishes the dosimetry of the ganglioside-IL-2 interaction. Since rIL-2 is administered systematically into stage IV melanoma patients, we have examined 45 tumor biopsies for GD(1b) content. The incidence of GD(1b) in tumor biopsies is 51%. We postulate that GD(1b) associated on the tumor or in the circulation of cancer patients may bind to rIL-2 and prevent the availability of rIL-2 to augment antitumor-immune response.

References

Nov 27, 1979·Biochemistry·B P PetersM Flashner
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·T M KloppelD J Morré
Aug 28, 1979·Biochemical and Biophysical Research Communications·P Ganguly, N G Fossett
Dec 14, 1978·Biochemical and Biophysical Research Communications·J PortoukalianJ F Doré
Jan 1, 1992·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·E M SchutterP Kenemans
Jun 15, 1991·Cancer·M H RavindranathR F Irie
Feb 15, 1989·Biochemical and Biophysical Research Communications·T KawaguchiK Takatsuki
May 15, 1989·European Journal of Biochemistry·H J SennW Gerok
Jan 1, 1987·Journal of the National Cancer Institute·T TsuchidaR F Irie
Jan 1, 1987·Methods in Enzymology·S Ladisch, B Gillard
Sep 1, 1987·European Journal of Biochemistry·N V ProkazovaL D Bergelson
May 21, 1984·FEBS Letters·J ParkerH Ankel
Apr 30, 1984·Biochemical and Biophysical Research Communications·J M CarubiaJ M Varga
Mar 10, 1994·Journal of Immunological Methods·M H RavindranathM C Graves
Oct 1, 1995·Immunology Today·L D Bergelson
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P FerroniP P Gazzaniga
Aug 15, 1996·The Biochemical Journal·J P ZanettaJ C Michalski
Oct 12, 2000·Biochemical and Biophysical Research Communications·Y InokiT Hamamoto

❮ Previous
Next ❯

Citations

Mar 27, 2007·Future Oncology·Mepur H RavindranathDonald L Morton
Aug 24, 2006·Biochimica Et Biophysica Acta·Bruno SéguiThierry Levade
Dec 4, 2013·FEBS Letters·Mahiuddin Ahmed, Nai-Kong V Cheung
Apr 9, 2005·International Journal of Cancer. Journal International Du Cancer·Mepur H RavindranathDonald L Morton
Feb 16, 2006·Journal of Neuroscience Research·Diego Gómez-NicolaManuel Nieto-Sampedro
Oct 22, 2003·Pathologie-biologie·I Popa, J Portoukalian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.