Interleukin-2 in the ontogeny of human lymphoid tissues

Immunobiology
B G BödekerP F Mühlradt

Abstract

Cells from different fetal and adult lymphoid organs were tested for the capacity to (a) react to the T-cell growth factor interleukin-2 (IL-2) and (b) to produce IL-2 under appropriate conditions. IL-2 was determined as growth promoting activity for mouse T blasts. Optimal conditions for IL-2 production were: 5 X 10(6) cells/ml; 4-6 micrograms mitogen, 24 h incubation time. Concanavalin A was preferable for fetal thymus, whereas phytohemagglutinin was the appropriate mitogen for lymphocytes from blood and lymph node. Fetal and adult spleen cells produced equal activities of IL-2 irrespective of the mitogen used. Cells from thymus and spleen of 17-21-week-old fetuses produced IL-2 activities like adult cells. Fetal liver cells from the same fetuses produced no IL-2. A comparison of IL-2 activities released from cells of 26 donors showed that within individual variations there was no decrease of the capacity to produce IL-2 with old age. An activity present in culture media of mitogen treated lymphocytes which increased thymidine uptake in fetal liver cells could be distinguished from IL-2 by its different molecular weight and by a heat treatment which abolished IL-2 but not fetal liver cell growth promoting activity. This latte...Continue Reading

References

Jan 1, 1981·Immunological Reviews·P ToivanenO Ruuskanen
Jan 1, 1981·Immunological Reviews·S Gillis, J Watson

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Citations

Jul 1, 1986·Journal of Reproductive Immunology·H BesslerM Djaldetti
Mar 1, 1991·The Journal of Pediatrics·C B Wilson
Aug 1, 1995·Immunology and Cell Biology·P von HoegenJ F Krowka
May 1, 1984·Scandinavian Journal of Immunology·G A GastlH H Peter
Nov 1, 1982·European Journal of Immunology·P F Mühlradt, H G Opitz
Jan 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Françoise MascartCamille Locht

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