Interleukin-2 secretion by human B lymphocytes occurs as a late event and requires additional stimulation after CD40 cross-linking

European Journal of Immunology
V KindlerR H Zubler

Abstract

While Epstein-Barr virus (EBV)-immortalized B cell lines have been shown to secrete interleukin (IL)-2 after stimulation with either teleocidin or phorbol myristate acetate (PMA) and ionomycin, experimental conditions leading to IL-2 production by normal human B cells have not been reported. In the present study we investigated various B cell activating conditions, including--by analogy to EBV-immortalized B lymphocytes--stimulation of B cells that are already proliferating (in cultures with IL-4 and immobilized anti-CD40 monoclonal antibody; the anti-CD40 system). This approach showed that B lymphocytes secreted IL-2 in the culture medium, but only if they were first activated for more than 24 h in the anti-CD40 system before exposure to PMA plus ionomycin. The production rate of IL-2 by B lymphocytes reached a maximum after 6 days of priming in such cultures followed by 48 h of stimulation with PMA plus ionomycin, corresponding to 7% or 15% of that of fresh CD4+ T cells activated, respectively, with phytohemagglutinin plus PMA, or with PMA plus ionomycin for 48 h. This IL-2 production could not be attributed to T cell contamination nor to EBV-infected B cells according to flow cytometric and reverse transcriptase-polymerase c...Continue Reading

References

May 7, 1992·Nature·R J ArmitageC R Maliszewski
Jan 1, 1992·Advances in Immunology·J Banchereau, F Rousset
Jan 1, 1991·The Journal of Experimental Medicine·S Ehlers, K A Smith
Jun 1, 1989·Cellular Immunology·D BenjaminM S Gilmore
Feb 3, 1994·Nature·E A Clark, J A Ledbetter

❮ Previous
Next ❯

Citations

Feb 6, 2002·Springer Seminars in Immunopathology·R H Zubler
Nov 26, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christian MuellerTerence R Flotte
Jul 6, 2001·Scandinavian Journal of Immunology·N PorakishviliP Youinou
Oct 23, 1998·The Journal of Experimental Medicine·V KouskoffD Nemazee
Apr 29, 1998·Immunological Reviews·F E LundM C Howard
Jun 22, 2006·International Journal of Hematology·Alana A Kennedy-NasserCliona M Rooney
Aug 3, 2002·Leukemia & Lymphoma·Brian F SkinniderTak W Mak
Jun 26, 2007·Scandinavian Journal of Immunology·S AmuM Brisslert
Feb 20, 2007·Protein Engineering, Design & Selection : PEDS·J Thusberg, M Vihinen
Jan 23, 1999·International Reviews of Immunology·P Chomarat, J Banchereau
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefania AmorosiClaudio Pignata
Nov 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Liliana BusconiAnn Marshak-Rothstein
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hadas SchoriMichal Schwartz
Dec 20, 2019·Cancer Immunology Research·Suresh de SilvaTaylor H Schreiber

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.