Interleukin 22 attenuated angiotensin II induced acute lung injury through inhibiting the apoptosis of pulmonary microvascular endothelial cells

Scientific Reports
Zhiyong WuBowen Li

Abstract

Apoptosis of pulmonary microvascular endothelial cells (PMVECs) was considered to be closely related to the pathogenesis of acute lung injury (ALI). We aim to investigate whether IL-22 plays protective roles in lung injury through inhibiting the apoptosis of PMVECs. ALI model was induced through subcutaneous infusion of angiotensin II (Ang II). Lung injury and infiltration of inflammatory cells were evaluated by determining the PaO2/FiO2, calculation of dry to weight ratio in lung, and immunohistochemisty analysis. Apoptosis of PMVECs was determined using TUNEL assay and flow cytometry, respectively. Immunofluorescence and Western blot analysis were used to determine the expression and localization of STAT3, as well as the nucleus transmission of STAT3 from cytoplasm after IL22 treatment. Pathological findings showed ALI was induced 1 week after AngII infusion. IL22 inhibited the AngII-induced ALI, attenuated the edema in lung and the infiltration of inflammatory cells. Also, it contributed to the apoptosis of PMVECs induced by AngII. Meanwhile, significant increase was noticed in the expression of STAT3, phosphorylation of Y705-STAT3, and migration from cytoplasm to the nucleus after IL-22 treatment (P < 0.05). The activation ...Continue Reading

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Citations

Nov 28, 2017·Current Opinion in Critical Care·Lucy K ReissStefan Uhlig
Dec 13, 2018·Angiogenesis·Nicholas J ProtopsaltisNapoleone Ferrara
Jun 7, 2020·Critical Care : the Official Journal of the Critical Care Forum·Zhiyong WuXiaoyang Zhou
Apr 6, 2018·Oxidative Medicine and Cellular Longevity·Ana Beatriz Farias de SouzaFrank Silva Bezerra
Aug 3, 2021·Molecular and Cellular Biochemistry·Xiaolei GongHaibo Zhang

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Methods Mentioned

BETA
dissection
Protein Assay
flow cytometry
MDA

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