Interleukin-29 binds to melanoma cells inducing Jak-STAT signal transduction and apoptosis.

Molecular Cancer Therapeutics
Kristan D GuenterbergWilliam E Carson

Abstract

Interleukin-29 (IL-29) is a member of the type III IFN family that has been shown to have antiviral activity and to inhibit cell growth. Melanoma cell lines were tested for expression of the IL-29 receptor (IL-29R) and their response to IL-29. Expression of IL-28R1 and IL-10R2, components of IL-29R, was evaluated using reverse transcription-PCR. A combination of immunoblot analysis and flow cytometry was used to evaluate IL-29-induced signal transduction. U133 Plus 2.0 Arrays and real-time PCR were used to evaluate gene expression. Apoptosis was measured using Annexin V/propridium iodide staining. In situ PCR for IL-29R was done on paraffin-embedded melanoma tumors. Both IL-28R1 and IL-10R2 were expressed on the A375, 1106 MEL, Hs294T, 18105 MEL, MEL 39, SK MEL 5, and F01 cell lines. Incubation of melanoma cell lines with IL-29 (10-1,000 ng/mL) led to phosphorylation of signal transducer and activator of transcription 1 (STAT1) and STAT2. Microarray analysis and quantitative reverse transcription-PCR showed a marked increase in transcripts of IFN-regulated genes after treatment with IL-29. In the F01 cell line, bortezomib-induced and temozolomide-induced apoptosis was synergistically enhanced following the addition of IL-29. In...Continue Reading

References

Mar 20, 2002·Cytokine & Growth Factor Reviews·Filippo BelardelliJohn M Kirkwood
Dec 7, 2002·Nature Immunology·Paul SheppardKevin M Klucher
Dec 17, 2002·Nature Immunology·Sergei V KotenkoRaymond P Donnelly
Jun 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gregory B LesinskiWilliam E Carson
Sep 1, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Barbara L AndersenWilliam E Carson
Sep 2, 2004·Journal of the National Cancer Institute·Gregory B LesinskiWilliam E Carson
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lyudmila BurdelyaHua Yu
Apr 27, 2005·Biochemical and Biophysical Research Communications·Stephan BrandChristoph J Auernhammer
Jul 30, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Stephan BrandJulia Dambacher
Sep 20, 2005·Current Biology : CB·Claudia WellbrockManfred Schartl
Mar 17, 2006·Cancer Research·Tong-xin XieSuyun Huang
May 3, 2006·Biochemical and Biophysical Research Communications·Kathrin ZitzmannChristoph J Auernhammer
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Atsuko SatoTakashi Murakami
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julie M RodaWilliam E Carson
Sep 29, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sean E DoyleKevin M Klucher
Mar 16, 2007·Genes and Immunity·W J JordanG Gallagher
Mar 21, 2007·Biochimie·Gilles Uzé, Danièle Monneron
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gregory B LesinskiWilliam E Carson
Oct 5, 2007·The Oncologist·Ian QuirtManya Charette
Oct 27, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Julie M RodaWilliam E Carson
Mar 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jason M ZimmererWilliam E Carson
Sep 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jason M ZimmererWilliam E Carson
Mar 26, 2009·BioTechniques·Gerard NuovoThomas Schmittgen
Apr 28, 2009·Cancer Immunology, Immunotherapy : CII·Gregory B LesinskiWilliam E Carson

❮ Previous
Next ❯

Citations

Jul 22, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Håkan C Steen, Ana M Gamero
Oct 2, 2012·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Yasuhiro TezukaTakashi Murakami
Aug 16, 2011·Current Opinion in Immunology·Sergei V Kotenko
Oct 12, 2010·Journal of Molecular Biology·Zachary J MiknisAlexander Wlodawer
May 31, 2012·Journal of Cellular Biochemistry·Yun-Fei XieBing-Ren Huang
Jul 30, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kayhan ErturkSezai Vatansever
Mar 8, 2011·The Journal of Allergy and Clinical Immunology·Mübeccel AkdisCezmi A Akdis
Oct 21, 2011·Clinical & Developmental Immunology·Masatoshi TagawaHideaki Shimada
Jul 20, 2016·Mediators of Inflammation·Lingxiao XuFang Wang
Sep 18, 2016·Critical Reviews in Oncology/hematology·Noah E KelmYujiang Fang
Jul 29, 2017·Immunological Investigations·Yoshitaka HosokawaTakashi Matsuo
Feb 11, 2015·Journal of Innate Immunity·Andrew Stiff, William Carson
Oct 19, 2017·Cold Spring Harbor Perspectives in Biology·Xiaoting WangSascha Rutz
Apr 29, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shuo TianHong Chen
Oct 4, 2019·Journal of Cellular and Molecular Medicine·Jia-Min WangLin-Chong Su
Feb 16, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alena Cristina Jaime-RamirezWilliam E Carson
Mar 23, 2017·Frontiers in Immunology·Xu WangWen-Zhe Ho
Sep 30, 2016·Expert Opinion on Therapeutic Targets·Mohammed Eslam, Jacob George
Jul 6, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohammad MalekanFateme Sheida
Sep 5, 2019·Journal of Autoimmunity·Stelios Vlachiotis, Evangelos Andreakos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
Quanhai LiMasatoshi Tagawa
Biochemical and Biophysical Research Communications
Kathrin ZitzmannChristoph J Auernhammer
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Eugenia MagrachevaAlexander Zdanov
© 2021 Meta ULC. All rights reserved