Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors.

Immunological Investigations
Yuji ShimizuH Saito

Abstract

Although IL-3 is commonly used for culture of human progenitor-derived mast cells together with Stem cell factor (SCF) and IL-6, the effect of IL-3 on human mast cell differentiation has not been well elucidated. Human bone marrow CD34+ progenitors were cultured for up to 12 weeks in the presence of rhSCF and rhIL-6 either with rhIL-3 (IL-3 (+)) or without rhIL-3 (IL-3 (-)) for the initial 1-week of culture. Total cell number increased at 2 weeks in IL-3 (+), as compared to IL-3 (-), but changes in the appearance of mast cells were delayed. When IL-3 was present for the initial 1-week culture, granules looked more mature with IL-3 than without IL-3. However, tryptase and chymase contents, and surface antigen expression (CD18, CD51, CD54, and CD117) were not altered by IL-3. Surface expression and mRNA level of FcepsilonRIalpha and histamine release by crosslinking of FcepsilonRIalpha did not differ from one preparation to the next. GeneChip analysis revealed that no significant differences were observed between IL-3 (+) and IL-3 (-) cells either when inactivated or activated by aggregation of FcepsilonRIalpha. These findings indicate that initial incubation of human bone marrow CD34+ progenitors with IL-3 does not affect the di...Continue Reading

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Citations

Oct 21, 2010·Human Reproduction Update·F M MenziesS M Nelson
Jun 3, 2008·British Journal of Haematology·Valeska HeibMichael Stassen
Jul 27, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Elaine Zayas Marcelino da SilvaConstance Oliver
Sep 30, 2017·Cell Death and Differentiation·Ramona ReinhartThomas Kaufmann
Nov 18, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jenny PaupertBéatrice Cousin
Aug 20, 2019·Immunological Investigations·Kate Rittenhouse-Olson
Aug 10, 2017·Blood·Joakim S DahlinGunnar Nilsson
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·John T SchroederAnja P Bieneman
Oct 29, 2009·Immunological Investigations·Paulo R V FallavenaClarice S Alho
May 15, 2018·Frontiers in Immunology·Heather L CaslinJohn J Ryan
Jan 1, 2018·Cell Death and Differentiation·Ramona ReinhartThomas Kaufmann
Sep 11, 2021·The Journal of Allergy and Clinical Immunology·Yuzhi YinDean D Metcalfe

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Methods Mentioned

BETA
flow cytometry
electron microscopy
ELISA
FACS

Software Mentioned

GeneChip
Primer Express
WinMDI

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