Interleukin-32 plays an essential role in human calcified aortic valve cells

European Cytokine Network
Chung-Lin TsaiYi-Ying Wu

Abstract

Interleukin-32 (IL-32) is an inflammatory cytokine produced mainly by T, natural killer, and epithelial cells. Previous studies on IL-32 have primarily investigated its proinflammatory properties. The IL-32 also has been described as an activator of the p38 mitogen-activated protein kinase (MAPK) and NF-κB, and induces several cytokines. In this study, we hypothesized that the inflammatory regulators NF-κB, MAP kinase, STAT1, and STAT3 are associated with the expression of the IL-32 protein in human calcified aortic valve cells. This study comprised aortic valve sclerotic patients and control group patients without calcified aortic valve. Increased IL-32 expression in calcified aortic valvular tissue was shown by immunohistochemical staining and western blotting. There was an increase in NF-κB p65 level, p-ERK, p-JNK, and p-p38 MAPK activation underlying IL-32 expression in the study. The level of p-STAT3 but not p-STAT1 was found to be increased in calcified aortic valve tissue. In cultured primary human aortic valve interstitial cells, inhibition of NF-κB or MAPK kinase pathways results in a decrease of IL-32 expression. Treatment of recombinant IL-32 induced the levels of TNF-α, IL-6, IL-1β, and IL-8. Our findings demonstrat...Continue Reading

Citations

Apr 17, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Michael A RaddatzW David Merryman
Jan 16, 2020·International Journal of Molecular Sciences·Praveen Kumar NattramilarasuJörg-Dieter Schulzke
May 6, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Michael A RaddatzW David Merryman
Nov 21, 2021·Journal of Clinical Laboratory Analysis·Susu JinMinghua Jiang
Dec 23, 2021·ESC Heart Failure·Mareike BarthPayam Akhyari

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