Interleukin 33 and interleukin 4 regulate interleukin 31 gene expression and secretion from human laboratory of allergic diseases 2 mast cells stimulated by substance P and/or immunoglobulin E

Allergy and Asthma Proceedings
Anastasia I PetraTheoharis C Theoharides

Abstract

Cytokine interleukin (IL) 31 has emerged as an important component of allergic and inflammatory diseases associated with pruritus, such as atopic dermatitis (AD) and mastocytosis. Mast cells (MC) are stimulated by allergic and nonallergic triggers, and play a critical role in such diseases by secreting histamine and tryptase as well as cytokines and chemokines. IL-33 has been reported to augment MC responses, but its effect on secretion of IL-31 is not known. To investigate whether IL-33 can stimulate the secretion of IL-31 from cultured human MCs and whether this response is augmented by either the neuropeptide substance P (SP) or immunoglobulin E (IgE) and anti-IgE in the absence or presence of IL-4. Laboratory of Allergic Diseases (LAD2) human MCs were cultured in StemProH-34 SFM medium supplemented by stem cell factor and were stimulated either with IL-33 (10 ng /mL) or SP (2 μM), or preincubated with IgE (1 μg/mL) overnight, and then stimulated with anti-IgE (1 μg/mL) for 24 hours. IL-31 gene expression was measured by quantitative polymerase chain reaction, and protein was measured by enzyme-linked immunosorbent assay. IL-33 (10 ng/mL) induces IL-31 gene expression, synthesis, and secretion from LAD2 cells in the absence ...Continue Reading

Citations

Mar 19, 2019·Expert Review of Clinical Immunology·Theoharis C TheoharidesHuali Ren
Oct 4, 2020·Nutrients·Joseph MuñozBahram H Arjmandi
Feb 20, 2020·International Journal of Molecular Sciences·Massimo De MartinisLia Ginaldi
Aug 21, 2019·Frontiers in Cellular Neuroscience·Theoharis C TheoharidesMona Bawazeer
Oct 18, 2019·Frontiers in Cellular Neuroscience·Hanna Siiskonen, Ilkka Harvima
May 27, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Xi ChenZongxiang Tang
May 21, 2020·Immunity·Fang Wang, Brian S Kim
Feb 26, 2021·Allergy·Angeliki DatsiJoerg Buddenkotte
Jul 21, 2020·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Theoharis C Theoharides
Apr 4, 2021·The FEBS Journal·Jennifer Fan, Santosh K Mishra
Apr 7, 2020·The Journal of Investigative Dermatology·Fang WangBrian S Kim
Jun 1, 2021·Frontiers in Cell and Developmental Biology·Gandhi F Pavón-RomeroLuis M Terán
Feb 11, 2019·International Archives of Allergy and Immunology·Dagmar SimonHans-Uwe Simon

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