Interleukin-33 enhanced the migration and invasiveness of human lung cancer cells

OncoTargets and Therapy
Zhiping YangYufen Xu

Abstract

Interleukin-33 (IL-33), belonging to IL-1 family cytokines, has been reported to participate in cancer growth and metastasis. The clinical values of IL-33 in lung cancer have been previously investigated. We aimed to elucidate the probable role of IL-33 in the migration and invasion of lung cancer cells. Cell migration and invasiveness were tested by Transwell assay. Western blotting analysis was performed to detect protein expression. We found that IL-33 treatment in human lung A549 cells dose-dependently enhanced their migratory and invasive ability, accompanied by elevated expression of matrix metallo-proteinase (MMP) 2 and MMP9. Meanwhile, IL-33-induced cell migration and invasion were significantly abolished by small interfering RNA-targeting ST2, the specific receptor of IL-33. Furthermore, IL-33 exposure induced the phosphorylation of AKT. Pretreatment with an AKT inhibitor LY294002 markedly attenuated IL-33-induced cell migration and invasion. IL-33/ST2 promoted the migration and invasiveness of lung cancer cells through AKT pathway. Our findings strongly suggest that IL-33 may serve as a promising therapeutic strategy for lung cancer.

Citations

Oct 9, 2018·Cell Adhesion & Migration·Christian MayrTobias Kiesslich
May 28, 2019·Biomolecules·Marco CasciaroSebastiano Gangemi
Jun 25, 2019·Frontiers in Immunology·Kevin J BakerElizabeth Brint
Jan 11, 2020·Frontiers in Oncology·Saray Quintero-FabiánMaría Elizbeth Alvarez-Sánchez
Sep 13, 2018·International Journal of Molecular Sciences·Kristen M LarsenMaria Marjorette O Peña
Nov 30, 2018·Frontiers in Immunology·Claudia AfferniGiovanna Schiavoni
Aug 13, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuhiko SugaAtsushi Kumanogoh

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection

Software Mentioned

GraphPad
GraphPad Prism
ImageJ

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.