Interleukin-33 Predicts Poor Prognosis and Promotes Renal Cell Carcinoma Cell Growth Through its Receptor ST2 and the JNK Signaling Pathway

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Chang-Wen WuAn-Yi Zhu

Abstract

Renal cell carcinoma (RCC) is currently the ninth most common cancer in men. Interleukin (IL)-33 expression has previously been associated with a number of cancers; however, its biological role in RCC is poorly understood. In this study, we sought to elucidate the role of IL-33 in RCC. Serum IL-33 levels were measured by ELISA. IL-33 expression in clinical RCC samples was examined by immunocytochemistry. The proliferation and apoptosis rate of RCC were determined by CCK8 and flow cytometry. Mcl1 and Bcl-2 expression were measured by quantitative real-time PCR and western blotting. JNK expression were measured by western blotting and flow cytometry. The in vivo role of IL-33 in RCC tumorigenesis was examined by animal models. We found that increased expression of IL-33 in RCC was associated with tumor-lymph node-metastasis (TNM) stage and inversely correlated with prognosis. IL-33 enhances RCC cell growth in vivo and stimulates RCC cell proliferation and prevents chemotherapy-induced tumor apoptosis in vitro. Furthermore, we demonstrated that IL-33 promotes RCC cell proliferation and chemotherapy resistance via its receptor ST2 and the JNK signaling activation in tumor cells. Our findings suggest that targeting IL-33/ST2 and JNK...Continue Reading

Citations

Sep 27, 2018·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jaewoo HongP Charles Lin
Feb 17, 2019·Medicina·Alessandro TonacciSebastiano Gangemi
Jul 29, 2020·Frontiers in Immunology·Moritz F EissmannMatthias Ernst
Nov 30, 2018·Frontiers in Immunology·Claudia AfferniGiovanna Schiavoni
Jan 31, 2019·Proceedings of the National Academy of Sciences of the United States of America·Amir H AmeriShadmehr Demehri
Jun 17, 2021·European Journal of Immunology·Wenyi JiangYi Zhang
Apr 1, 2019·Dermatology : International Journal for Clinical and Investigative Dermatology·Fuhan YangYuemei Ma

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