Interleukin 37 Expression Inhibits STAT3 to Suppress the Proliferation and Invasion of Human Cervical Cancer Cells

Journal of Cancer
Sen WangHui Xiong

Abstract

The most recently discovered cytokine interleukin 37 (IL-37) received growing attention. Its function on tumor is largely unknown. Here, we investigated the biological function of IL-37 on cervical cancer (CC). Materials and methods : HPV(+) Hela cells and HPV(-) C33A cells were used. RT-qPCR was performed to detect the transcription of IL-37, STAT3, TNF-αand IL-1β. Western blotting was used for protein detection. CCK-8 assay and transwell assay were employed for cell proliferation and invasion detection, respectively. Results : Successful gene transfection of IL-37 suppressed the proliferation and invasion of CC. Interestingly, IL-37 showed higher anticancer ability in HPV(+) Hela cells than that in HPV(-) C33A cells. Then, the molecular mechanism of IL-37 anticancer was explored. Firstly, we found that IL-37 inhibited STAT3 expression at both mRNA and protein levels. IL-37 also down regulated the phosphorylation of STAT3. Secondly, blockage of STAT3 using siRNAs reduced significantly the ability of IL-37 to suppress cell proliferation and invasion. Thirdly, STAT3 knockdown reduced markedly the inhibition of IL-37 on the transcription of tumor-derived TNF-α and IL-1β, indicating the contribution of STAT3 for the cancer associa...Continue Reading

Citations

Jan 23, 2016·Journal of Experimental & Clinical Cancer Research : CR·Guanqun GeYan Xue
Jun 3, 2016·Medical Oncology·Vivi A DingYujiang Fang
Apr 4, 2018·Archives of Gynecology and Obstetrics·Peng LiSen Wang
Mar 4, 2020·Journal of Leukocyte Biology·Yu MeiHaiyan Liu
Jul 14, 2016·Biological Chemistry·Javier Mora, Andreas Weigert
May 29, 2018·BioMed Research International·Hailin JiaBo Han
Jun 2, 2018·Journal of Immunology Research·Marconi Rego BarrosAntonio Carlos de Freitas
May 10, 2019·Oncoimmunology·Xiaolin WangWenling Han
Sep 28, 2017·Cell Biology International·Rafael P de CamposMárcia R Wink
Jun 25, 2019·Frontiers in Immunology·Kevin J BakerElizabeth Brint
Mar 23, 2017·World Journal of Gastroenterology : WJG·Jie WangJian-Xin Jiang
Apr 1, 2019·Cancer Reports·Yu Mei, Haiyan Liu
Mar 24, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Xishuang WangXiaoyan Wang
Mar 23, 2021·Frontiers in Immunology·Steffeni MountfordPhilip Bufler
Apr 29, 2021·Cancer Management and Research·Xiaonan WeiChengming Sun

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR

Software Mentioned

SPSS13

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Jing WangZehua Wang
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Yunlai ZhiKun Yang
© 2021 Meta ULC. All rights reserved