Interleukin-37 suppresses tumor growth through inhibition of angiogenesis in non-small cell lung cancer

Journal of Experimental & Clinical Cancer Research : CR
Guanqun GeYan Xue

Abstract

Interleukin-37 (IL-37), a newly identified member of the IL-1 family, has been known to play an immunosuppressive role in a variety of inflammatory disorders, but whether it participates in the regulation of pathogenesis of non-small cell lung cancer (NSCLC) has not been investigated. Real-time polymerase chain reaction (PCR), western blotting, and immunohistochemical staining were employed to detect IL-37 expression in NSCLC tissues and corresponding adjacent tissues. The correlations between IL-37 expression and clinicopathological characteristics, prognosis were analyzed. Stable clone with overexpression of IL-37 was generated in H1299 cell lines. Cell growth, cell cycle and cell apoptosis assays were carried out for detecting proliferation and apoptosis of H1299 cells. The effects of IL-37 on NSCLC progression in vivo was performed in a xenografted lung tumor model in nude mice. The concentrations of IL-37 and VEGF in the s growth medium supernatants were quantified by ELISA. The antiangiogenic effect of IL-37 on HUVEC was measured by tube formation assay. IL-37 mRNA and protein expressions were significantly decreased in NSCLC tissues, and decreased intratumoral IL-37 expression was significantly associated with tumor stat...Continue Reading

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Citations

Jun 12, 2016·Journal of Experimental & Clinical Cancer Research : CR·Jie TangZekuan Xu
Jul 26, 2016·Disease Markers·Ángela MarrugalIrene Ferrer
Nov 7, 2016·Biochemical and Biophysical Research Communications·Mengmeng ZhaoTianshu Yang
Dec 7, 2016·Modern Rheumatology·Jatin SharmaC Subathra Devi
Jul 14, 2016·Biological Chemistry·Javier Mora, Andreas Weigert
Dec 2, 2017·Liver International : Official Journal of the International Association for the Study of the Liver·Felix GrabherrHerbert Tilg
Mar 4, 2020·Journal of Leukocyte Biology·Yu MeiHaiyan Liu
Apr 27, 2019·Journal of Experimental & Clinical Cancer Research : CR·Ting WangLi Cai
Apr 12, 2016·European Journal of Immunology·Charles A DinarelloPhilip Bufler
Apr 1, 2019·Cancer Reports·Yu Mei, Haiyan Liu
May 29, 2018·BioMed Research International·Hailin JiaBo Han
Mar 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Elan Z EisenmesserCharles A Dinarello
Sep 14, 2019·Nature Reviews. Rheumatology·Charles Anthony Dinarello
Mar 29, 2020·Frontiers in Oncology·Poonam SarodeRajkumar Savai

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Methods Mentioned

BETA
PCR
ELISA
flow cytometry
FACS
xenograft

Software Mentioned

SPSS
- PC
Flowjo

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