Interleukin-4 as a bone regulatory factor: effects on murine osteoblast-like cells

Journal of Endocrinological Investigation
J A RianchoJ L Fernandez-Luna

Abstract

Bone remodelling is regulated at the local level by an incompletely elucidated cytokine network. In the present study we have determined the effect of interleukin-4 (IL-4), a cytokine produced by T lymphocytes and other cells, on the activity of murine osteoblasts. IL-4 (0.1-10 ng/ml) did not influence the proliferation rate of the osteoblast-like cell line MC3T3, but inhibited the expression of alkaline phosphatase. In long-term cultures supplemented with ascorbic acid and glycerophosphate such an effect was accompanied by a retardation of matrix mineralization. IL-4 also stimulated M-CSF expression by MC3T3 cells, both at the RNA and bioactivity levels. However, no stimulation of IL-1, IL-6, GM-CSF or PGE2 production was observed. An IL-4-induced inhibition of alkaline phosphatase expression and retardation of mineralization was also found in cultures of primary osteoblast-like cells isolated from neonatal mice calvariae. These results suggest that IL-4, probably released by cells within the bone marrow, may locally influence the activity of bone-forming cells.

References

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Citations

Jun 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Adrian H Chester, Patricia M Taylor
Dec 20, 2020·Journal of Biomedical Materials Research. Part a·Jukka PajarinenStuart B Goodman
Apr 4, 2021·International Journal of Molecular Sciences·Dulshara Sachini AmarasekaraJaerang Rho
Dec 2, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Robert F KleesGeorge E Plopper
Sep 30, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiangjiao YiZhenqiang Yao

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