Interleukin-4 induces both IgG4 and IgE secretion by peripheral blood B cells

Journal of Clinical Laboratory Analysis
H G Nüsslein, H L Spiegelberg

Abstract

Peripheral blood mononuclear cells (PBMCs) from healthy nonallergic donors were cultured with recombinant interleukin-4 (rIL-4), and the Ig of different isotypes was quantitated in the culture supernatants by radioimmunoassays. Recombinant IL-4 induced IgG4 and IgE secretion in a dose-dependent manner, whereas it had no consistent effect on the secretion of the other isotypes. In the absence of rIL-4, B cells in the PBMC preparations secreted less than 1 ng IgE/ml and a mean of 5 ng IgG4/ml. In the presence of the optimal dose of 100 U rIL-4/ml, PBMCs from five donors secreted a mean +/- SEM of 37 +/- 8 ng IgE/ml and 66 +/- 25 ng IgG4/ml. In kinetic studies, no IgG4 or IgE secretion was detected during the first 5 days of culture, and approximately 50% of the IgG4 and IgE secreted by day 15 was detected in supernatants on day 7. Cycloheximide, actinomycin-D, and mytomycin-C completely inhibited the rIL-4-induced IgG4 and IgE secretion, indicating that de novo protein, RNA, and DNA synthesis was required. As shown by Percoll buoyant density centrifugation, rIL-4 induced B cells in the high-density fraction to secrete IgG4 and IgE, whereas it inhibited spontaneous IgG4 secretion by low-density B cells. Interferon-gamma inhibited ...Continue Reading

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Citations

Nov 1, 1993·Journal of Clinical Immunology·H G NüssleinM Gramatzki
May 20, 1999·International Journal for Parasitology·S ZhangG J Weil
Feb 1, 1994·The Journal of Clinical Investigation·H L SpiegelbergA H Lucas
Apr 1, 1994·The Veterinary Quarterly·J Buijs, F van Knapen
Jun 17, 2011·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·Saroochi AgarwalVenu Gangur
Nov 1, 1994·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F H KleblH G Nüsslein
Aug 25, 2020·Journal of Experimental & Clinical Cancer Research : CR·Xiang QingTian-Sheng Wang
Nov 1, 1992·Clinical and Experimental Immunology·H G NüssleinJ R Kalden
Aug 1, 1992·Clinical and Experimental Immunology·C Ninomiya, H L Spiegelberg
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C GrüberR C Aalberse
Mar 1, 1994·The Journal of Allergy and Clinical Immunology·H Brummer-KorvenkontioT Palosuo

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